A. S. Dingens

Mapping the neutralizing specificity of human anti-HIV serum by deep mutational scanning

This study maps the neutralizing specificity of human anti-HIV serum using deep mutational scanning to identify Env mutations affecting neutralization by polyclonal sera.

Antibodies to the SARS-CoV-2 receptor-binding domain that maximize breadth and resistance to viral escape

This study identifies antibodies against SARS-CoV-2 that balance neutralization potency and breadth, with some showing high resistance to viral escape.

Functional development of a V3-specific broadly neutralizing antibody isolated from a case of HIV superinfection

This study characterizes the development of a V3-specific broadly neutralizing antibody from an HIV superinfection case, revealing unique epitope recognition and escape mechanisms.

Complete map of SARS-CoV-2 RBD mutations that escape the monoclonal antibody LY-CoV555 and its cocktail with LY-CoV016

The paper maps SARS-CoV-2 RBD mutations that escape LY-CoV555 and its cocktail with LY-CoV016, identifying escape mutations in circulating variants and suggesting epitope diversification for improved antibody resilience.

Genetic and structural basis for recognition of SARS-CoV-2 spike protein by a two-antibody cocktail

This paper determines the structures of two antibodies in the AZD7442 cocktail that neutralize SARS-CoV-2 and identifies critical residues for neutralization and viral escape.

Prospective mapping of viral mutations that escape antibodies used to treat COVID-19

The paper maps viral mutations in SARS-CoV-2 that escape two therapeutic antibodies, identifying escape mutations present in circulating strains.

High-resolution mapping of the neutralizing and binding specificities of polyclonal rabbit serum elicited by HIV Env trimer immunization

This study maps the neutralizing and binding specificities of polyclonal rabbit serum against HIV Env trimers using mutational antigenic profiling and EMPEM.

Complete mapping of mutations to the SARS-CoV-2 spike receptor-binding domain that escape antibody recognition

This study maps mutations in the SARS-CoV-2 spike RBD that escape antibody recognition, revealing escape mutation patterns and enabling the design of antibody cocktails.

Protocol and reagents for pseudotyping lentiviral particles with SARS-CoV-2 Spike protein for neutralization assays

This paper provides a protocol for pseudotyping lentiviral particles with SARS-CoV-2 Spike protein to measure neutralizing antibody activity in a luciferase-based assay.

APOBEC3C tandem domain proteins create super restriction factors against HIV-1

The paper shows that synthetic tandem domain APOBEC3C proteins create more potent antiviral factors against HIV-1 than native A3C, with increased resistance to viral antagonism.

Identification of HIV-1 Envelope Mutations that Enhance Entry Using Macaque CD4 and CCR5

This study identifies HIV-1 Env mutations that enhance entry into macaque cells using deep mutational scanning, finding mutations that increase entry up to 38-fold and affect antibody sensitivity.

Massively parallel profiling of HIV-1 resistance to the fusion inhibitor enfuvirtide

This study identifies additional resistance mutations to enfuvirtide using deep mutational scanning, expanding the known mechanisms of resistance.

An antigenic atlas of HIV-1 escape from broadly neutralizing antibodies

This study maps how single amino-acid mutations in HIV-1 Env affect neutralization by nine broadly neutralizing antibodies, revealing escape mechanisms and informing vaccine and therapy design.

Complete functional mapping of infection- and vaccine-elicited antibodies against the fusion peptide of HIV

The paper maps functional epitopes of antibodies targeting HIV Env fusion peptide by analyzing mutations affecting neutralization, revealing differences between vaccine- and infection-elicited antibodies.

Mapping mutational effects along the evolutionary landscape of HIV envelope

The paper quantifies how amino-acid mutations affect HIV Env protein fitness across two diverged viral strains, finding most sites have conserved preferences but some show shifted mutational tolerance.

Comprehensive mapping of HIV-1 escape from a broadly neutralizing antibody

This study maps HIV-1 escape mutations from a broadly neutralizing antibody by quantifying the effect of all single amino-acid mutations on viral sensitivity.