N. P. King

Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV

This study develops MERS-CoV vaccines using a protein nanoparticle platform, showing they induce strong neutralizing antibodies and protect mice against MERS-CoV challenge.

Deep mutational scanning reveals functional constraints and antigenic variability of Lassa virus glycoprotein complex

A broadly generalizable stabilization strategy for sarbecovirus fusion machinery vaccines

This paper develops a stabilization strategy for sarbecovirus fusion machinery vaccines, showing broad reactivity and protection against SARS-CoV-2 variants.

Local structural flexibility drives oligomorphism in computationally designed protein assemblies

This study shows that structural flexibility in computationally designed protein assemblies leads to multiple architectures, expanding their functional possibilities.

Broad receptor tropism and immunogenicity of a clade 3 sarbecovirus

This study characterizes the receptor tropism and antigenicity of a clade 3 sarbecovirus, identifying broad ACE2 usage and cross-neutralizing antibodies with implications for vaccine design.

Rapid and automated design of two-component protein nanomaterials using ProteinMPNN

This study benchmarks ProteinMPNN against Rosetta in designing two-component protein nanomaterials, showing comparable success with significantly reduced computation and no manual refinement.

Saponin Nanoparticle Adjuvants Incorporating Toll-Like Receptor Agonists Drive Distinct Immune Signatures and Potent Vaccine Responses

This paper develops a modular saponin-based nanoparticle adjuvant platform incorporating TLR agonists, showing improved vaccine potency and breadth against COVID-19 and HIV in mice.

Antigen spacing on protein nanoparticles influences antibody responses to vaccination

This study shows that antigen spacing on protein nanoparticles affects antibody responses to vaccination, with decreased spacing improving hemagglutination inhibition and neutralization potency.

Combinatorial immune refocusing within the influenza hemagglutinin head elicits cross-neutralizing antibody responses

This study evaluates trihead nanoparticle immunogens to enhance cross-neutralizing antibody responses against influenza H1 viruses by combining immunofocusing techniques.

Computational design of non-porous, pH-responsive antibody nanoparticles

This paper designs pH-responsive antibody nanoparticles with three distinct protein components that disassemble at tunable pH levels, enabling targeted delivery of biologics.

Extremely potent pan-sarbecovirus neutralizing antibodies generated by immunization of macaques with an AS03-adjuvanted monovalent subunit vaccine against SARS-CoV-2

This study identifies potent pan-sarbecovirus neutralizing antibodies from vaccinated macaques, showing broad neutralization against SARS-CoV-2 variants and related viruses.

Broad and Durable Humoral Responses Following Single Hydrogel Immunization of SARS-CoV-2 Subunit Vaccine

This study develops a single-dose SARS-CoV-2 subunit vaccine using hydrogel technology that generates rapid, broad, and durable humoral immunity compared to traditional multi-dose regimens.

De novo designed ice-binding proteins from twist-constrained helices

This paper designs ice-binding proteins with controlled under-twisting of alpha-helices to enhance ice recrystallization inhibition.

Fast and versatile sequence-independent protein docking for nanomaterials design using RPXDock

RPXDock enables fast, flexible protein docking for symmetric architectures, supporting diverse applications in nanomaterial design.

Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains

This study identifies cryptic transmembrane domains in designed protein nanoparticles that hinder secretion and introduces a computational tool, Degreaser, to remove them while maintaining stability, improving secretion efficiency.

Robust deep learning based protein sequence design using ProteinMPNN

A deep learning method for protein sequence design shows high performance in both simulations and experiments, applicable to various design challenges.

Increasing computational protein design literacy through cohort-based learning for undergraduate students

This paper describes a remote cohort-based curriculum for undergraduate students to learn computational protein design, enhancing their research skills and connecting them with the lab.

Durable protection against SARS-CoV-2 Omicron induced by an adjuvanted subunit vaccine

This study shows that an adjuvanted subunit vaccine provides durable protection against SARS-CoV-2 Omicron in non-human primates, with strong neutralizing antibodies and memory T/B cells after booster doses.

Immunization with a self-assembling nanoparticle vaccine displaying EBV gH/gL protects humanized mice against lethal viral challenge

This study evaluates a nanoparticle vaccine displaying EBV gH/gL, showing it protects humanized mice against lethal EBV challenge, while monomeric gH/gL does not.

Distinct sensitivities to SARS-CoV-2 variants in vaccinated humans and mice

This study compares neutralizing antibody responses to SARS-CoV-2 variants in vaccinated humans and mice, finding that mice do not accurately reflect the breadth and potency seen in humans.

Qualification of ELISA and neutralization methodologies to measure SARS-CoV-2 humoral immunity using human clinical samples

This paper qualifies ELISA and neutralization assays to measure SARS-CoV-2 humoral immunity using clinical samples, evaluating antibody titers and functional neutralization capacity.

Structure-based design of stabilized recombinant influenza neuraminidase tetramers

This study designs stabilized recombinant influenza neuraminidase tetramers by uncovering structural principles and improving thermal stability and antibody affinity.

Stabilization of the SARS-CoV-2 Spike receptor-binding domain using deep mutational scanning and structure-based design

This paper designs stabilized SARS-CoV-2 RBD variants using deep mutational scanning and structure-based design, showing improved thermal stability and manufacturability for vaccine applications.

Elicitation of broadly protective sarbecovirus immunity by receptor-binding domain nanoparticle vaccines

This study shows that a SARS-CoV-2 RBD nanoparticle vaccine protects against SARS-CoV-2 and related sarbecoviruses, including variants, in mice and non-human primates.

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

The paper uses cryoEM to analyze polyclonal antibody responses to HIV Env immunogens in rhesus macaques, revealing structural differences in antibody binding and immunogenicity of engineered mutations and glycan sites.

Designed proteins assemble antibodies into modular nanocages

The paper designs modular nanocages that assemble antibodies into precise geometries, enhancing signaling and neutralization compared to free antibodies.

Immunofocusing and enhancing autologous Tier-2 HIV-1 neutralization by displaying Env trimers on two-component protein nanoparticles

This study shows that displaying HIV-1 Env trimers on two-component protein nanoparticles enhances autologous Tier-2 neutralizing antibody titers in rabbits.

Elicitation of potent neutralizing antibody responses by designed protein nanoparticle vaccines for SARS-CoV-2

This study designs SARS-CoV-2 nanoparticle vaccines that induce strong neutralizing antibodies in mice, with higher titers and lower doses than existing trimer vaccines.

Elicitation of broadly protective immunity to influenza by multivalent hemagglutinin nanoparticle vaccines

Nanoparticle immunogens with four seasonal influenza hemagglutinins elicit neutralizing antibodies against both the head and stem, providing broad protection against diverse influenza strains.

Protocol and reagents for pseudotyping lentiviral particles with SARS-CoV-2 Spike protein for neutralization assays

This paper provides a protocol for pseudotyping lentiviral particles with SARS-CoV-2 Spike protein to measure neutralizing antibody activity in a luciferase-based assay.