Author

M. Kopylov

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 2 Microbiology· 2 Biochemistry· 1 Immunology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Assay:cryo-EM· 2 Assay:electronic health records· 2 Research Focus:host-pathogen & immune response· 2 Organism:human· 2 Assay:proteomics· 2 Organism:bacteria (other)· 1 Research Focus:cell type annotation· 1

Frequent Co-Authors

E. T. Eng· 3 shared A. J. Noble· 2 shared B. Carragher· 2 shared C. S. Potter· 2 shared D. Bobe· 2 shared A. B. Massimi· 1 shared A. Celikgil· 1 shared A. de Marco· 1 shared

Activity by Year

2019-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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3 shared papersMicrobiology

E. T. Eng

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Heterologous Prime-Boost with Immunologically Orthogonal Protein Nanoparticles for Peptide Immunofocusing

S. Bhattacharya, M. C. Jenkins, P. Keshavarz-Joud, A. R. Bourque, K. White, A. M. A. Barkane, A. V. Bryksin, C. Hernandez, M. Kopylov, M. G. Finn

This paper develops a vaccine strategy using immunologically orthogonal protein nanoparticles to focus antibody responses on a specific SARS-CoV-2 RBD epitope.

bioRxiv · Feb 24, 2024Immunology

Square beams for optimal tiling in TEM

E. Y. D. Chua, L. M. Alink, M. Kopylov, F. Eisenstein, J. D. Johnston, A. de Marco

This paper introduces a square electron beam for TEM to improve tiling and reduce exposure unevenness in cryo-EM imaging.

bioRxiv · Jul 29, 2023 · PreprintBiophysics

Waffle Method: A general and flexible approach for FIB-milling small and anisotropically oriented samples

K. Kelley, A. M. Raczkowski, O. Klykov, P. Jaroenlak, D. Bobe, M. Kopylov, E. T. Eng, G. Bhabha, C. S. Potter, B. Carragher, A. J. Noble

The paper introduces the Waffle Method to improve cryo-FIB/SEM workflows for preparing complex biological samples for cryo-ET, demonstrating its effectiveness with various samples and hardware.

bioRxiv · Oct 28, 2020Microbiology

Time-resolved cryoEM using Spotiton

V. P. Dandey, W. C. Budell, H. Wei, D. Bobe, K. Maruthi, M. Kopylov, E. T. Eng, P. A. Kahn, J. E. Hinshaw, N. Kundu, C. M. Nimigean, C. Fan, N. Sukomon, S. Darst, R. Saecker, , B. Carragher

The paper introduces a method for preparing cryoEM grids to study short-lived molecular states by rapidly dispensing sample drops and vitrifying them.

Microscopy and Microanalysis · Jul 30, 2020Peer-reviewedBiophysics

Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis

N. G. Herrera, N. C. Morano, A. Celikgil, G. I. Georgiev, R. J. Malonis, J. H. Lee, K. Tong, O. Vergnolle, A. B. Massimi, L. Y. Yen, A. J. Noble, M. Kopylov, J. B. Bonanno, S. C. Garrett-Thomson, D. B. Hayes, , S. C. Almo

This study evaluates the production, characterization, and antigenicity of SARS-CoV-2 S protein constructs in two cell lines, showing high-quality yields and consistent behavior across assays.

bioRxiv · Jun 14, 2020Biochemistry

Ribosome dimerization prevents loss of essential ribosomal proteins during quiescence

H. Feaga, M. Kopylov, J. K. Kim, M. Jovanovic, J. Dworkin

This study shows that HPF in Bacillus subtilis prevents loss of essential ribosomal proteins during quiescence by mediating ribosome dimerization.

bioRxiv · Oct 16, 2019 · PreprintMicrobiology