The paper identifies ALCAM-CD6 co-stimulation as a pathway promoting IgG2b class-switching in lymph nodes during advanced inflammatory arthritis using spatial and single-cell transcriptomics.

bioRxiv · Oct 27, 2022 · PreprintImmunology

Aging and Obesity Prime the Methylome and Transcriptome of Adipose Stem Cells for Disease and Dysfunction

S. Xie, S. Choudhari, C.-L. Wu, K. Abramson, D. Corcoran, S. G. Gregory, J. M. Thimmapuram, F. Guilak, D. Little

This study identifies epigenetic and transcriptomic changes in adipose stem cells due to aging and obesity, revealing synergistic effects and potential disease-related regulators.

bioRxiv · Sep 26, 2022Cell Biology

A new transcriptional metastatic signature predicts survival in clear cell renal cell carcinoma

A. M. Alchahin, S. Mei, I. Tsea, T. Hirz, Y. Kfoury, D. Dahl, C.-L. Wu, A. O. Subtelny, S. Wu, D. T. Scadden, J. H. Shin, P. J. Saylor, D. B. Sykes, P. V. Kharchenko, N. Baryawno

The paper identifies a transcriptional signature in primary ccRCC tumors that predicts metastatic potential and patient survival.

bioRxiv · Jun 28, 2022Cancer Biology

A Murine Model of Abductor Insufficiency Accelerates the Development of Hip Osteoarthritis

M. B. Geary, C. A. Orner, H. Shammas, J. M. Reuter, A. E. Loiselle, B. D. Giordano, C.-L. Wu

This study shows that abductor injury in mice leads to hip osteoarthritis, with cartilage degeneration and collagen loss, suggesting a link between muscle pathology and OA development.

bioRxiv · May 22, 2022 · PreprintPathology

Synovial fibroblasts assume distinct functional identities and secrete R-spondin 2 to drive osteoarthritis

A. J. Knights, E. C. Farrell, O. M. Ellis, L. Lammlin, L. M. Junginger, P. M. Rzeczycki, R. F. Bergman, R. Pervez, M. Cruz, A. A. Samani, C.-L. Wu, K. D. Hankenson, T. Maerz

This study identifies distinct synovial fibroblast subsets in PTOA and shows that Prg4hi fibroblasts secrete Rspo2 to drive pathological crosstalk.

bioRxiv · May 6, 2022 · PreprintCell Biology

Integrated single-cell and spatial transcriptomic analyses unravel the heterogeneity of the prostate tumor microenvironment

T. Hirz, S. Mei, H. Sarkar, Y. Kfoury, S. Wu, B. M. Verhoeven, A. O. Subtelny, D. V. Zlatev, M. W. Wszolek, K. Salari, E. Murray, F. Chen, E. Z. Macosko, C.-L. Wu, D. T. Scadden, , D. B. Sykes

This study uses single-cell and spatial transcriptomic analyses to characterize the prostate tumor microenvironment, revealing immune suppression and high angiogenic activity.

bioRxiv · Mar 18, 2022 · PreprintCancer Biology

Prospective Isolation of Chondroprogenitors from Human iPSCs Based on Cell Surface Markers Identified using a CRISPR-Cas9-Generated Reporter

A. Dicks, C.-L. Wu, N. Steward, S. S. Adkar, C. A. Gersbach, F. Guilak

The paper identifies and isolates a novel chondroprogenitor subpopulation from hiPSCs using surface markers and CRISPR-Cas9 reporter technology, showing enhanced chondrogenic potential.

bioRxiv · Jun 19, 2019Cell Biology

Gene Therapy for Follistatin Mitigates Systemic Metabolic Inflammation and Post-Traumatic Osteoarthritis in High-Fat Diet-Induced Obese Mice

R. Tang, N. Harasymowicz, C.-L. Wu, K. Collins, Y.-R. Choi, S. Oswald, F. Guilak

This study shows that FST gene therapy reduces metabolic inflammation and post-traumatic OA in obese mice by improving mitochondrial function and adipose tissue browning.

bioRxiv · Apr 25, 2019Physiology