E. Gouaux

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses

The paper develops methods to image glutamatergic synapses in hippocampal tissue using cryo-electron tomography, enabling high-resolution visualization of synaptic structures.

Single molecule studies of the native hair cell mechanosensory transduction complex

This study optimizes single molecule assays to detect and quantify the native hair cell mechanosensory transduction complex at attomole levels.

Structure of C. elegans TMC-2 complex suggests roles of lipid-mediated subunit contacts in mechanosensory transduction

The paper presents the cryo-EM structure of the C. elegans TMC-2 complex and compares it to the TMC-1 complex to identify structural features involved in mechanosensory transduction.

Purification and biochemical analysis of native AMPA receptors from three different mammalian species

This study isolates and analyzes native AMPA receptors from pig, sheep, and cow brains, showing structural and biochemical analysis is feasible in ungulates, which are evolutionarily closer to humans than rodents.

Structure of C. elegans TMC-1 complex illuminates auditory mechanosensory transduction

The paper determines the structure of the C. elegans TMC-1 complex using cryo-EM, revealing its composition and mechanism of mechanosensory transduction.

Aminomethanesulfonic acid illuminates the boundary between full and partial agonists of the pentameric glycine receptor

This study investigates aminomethanesulfonic acid (AMS) as an agonist of the glycine receptor, showing it acts as a full agonist with high efficacy and provides insights into agonist efficacy mechanisms.

Conserved His-Gly motif of acid-sensing ion channels resides in a reentrant loop implicated in gating and ion selectivity

This study determines the structure of chicken ASIC1a in resting and desensitized states, revealing the role of the His-Gly motif in ion permeation and gating.

Chemical and structural investigation of the paroxetine-human serotonin transporter complex

The paper investigates the binding of paroxetine and its analogs to the serotonin transporter using structural and biochemical methods, revealing how they stabilize the outward-open conformation and inhibit serotonin transport.

Divalent cation and chloride ion sites of chicken acid sensing ion channel 1a elucidated by x-ray crystallography

This study identifies ion binding sites in chicken ASIC1a using X-ray crystallography, revealing cation and anion sites across different functional states and their roles in proton-gating modulation.

Mechanisms for zinc and proton inhibition of the GluN1/GluN2A NMDA receptor

This study uses cryo-EM to determine the structure of the GluN1/GluN2A NMDAR under varying zinc and proton concentrations, revealing how zinc binding affects channel gating.

Thermostabilization and purification of the human dopamine transporter (hDAT) in an inhibitor and allosteric ligand bound conformation

This study identifies a thermostable variant of the human dopamine transporter (hDAT) and describes a method to purify it in an inhibitor and allosteric ligand bound conformation.

Expression and purification of a functional heteromeric GABAA receptor for structural studies

This paper describes methods to express and purify functional GABAA receptors for structural studies, identifying a minimal construct that retains agonist binding activity.

Structural basis for recognition of diverse antidepressants by the human serotonin transporter

The paper reports x-ray structures of the human serotonin transporter bound to antidepressants, explaining drug binding and selectivity mechanisms.

Activation and desensitization mechanism of AMPA receptor-TARP complex by cryo-EM

The paper determines the structure of the AMPA receptor-TARP complex using cryo-EM, revealing activation and desensitization mechanisms.