F. M. Bosisio

NK cells contribute to resistance to anti-PD1 therapy in immune-excluded melanomas

The paper identifies NK cells as contributors to resistance to anti-PD1 therapy in immune-excluded melanomas using single-cell RNA sequencing and spatial proteomics.

An autophagy program that promotes T cell egress from the lymph node controls responses to immune checkpoint blockade

This study shows that autophagy in lymphatic endothelial cells controls T cell egress from lymph nodes, which is critical for immune checkpoint therapy effectiveness.

Single-cell molecular profiling using ex vivo functional readouts fuels precision oncology in glioblastoma

The paper introduces PROSPERO, a single-cell assay to evaluate therapy response in glioblastoma by measuring molecular drug responses in fresh tumor samples, revealing limited therapeutic capacity and cellular plasticity.

Anticancer immunotherapies transition postcapillary venules into high-endothelial venules that generate TCF1+ T lymphocyte niches through a feed-forward loop

This paper shows that antiangiogenic immunotherapies convert postcapillary venules into high-endothelial venules that support TCF1+ T cell niches through a feed-forward loop involving CD8 and NK cells.

Immunogenomic, single-cell and spatial dissection of CD8+T cell exhaustion reveals critical determinants of cancer immunotherapy

This study identifies distinct CD8+T cell states in cancer patients using multi-omics data, showing that dysfunctional states resist immunotherapy while exhausted states can be targeted with specific treatments.

NOT ALL ANTIBODIES HAVE BEEN CREATED EQUAL: Antibodies validated for routinely processed tissue seldom apply to frozen tissue sections.

The paper validates 126 antibodies on frozen sections and finds that only 30% work across all fixations, highlighting the need for context-specific validation.

A fully defined pluripotent stem cell derived multi-liver-cell model for steatohepatitis and fibrosis

The paper develops a co-culture system of pluripotent stem cell-derived liver cells to model NASH-induced steatosis, inflammation, and fibrosis.

Activation of the Integrated Stress Response in drug-tolerant melanoma cells confers vulnerability to mitoribosome-targeting antibiotics.

This study shows that activating the Integrated Stress Response in drug-tolerant melanoma cells makes them vulnerable to mitoribosome-targeting antibiotics, offering a new therapeutic strategy.

A Pan-Cancer Blueprint of the Heterogeneous Tumour Microenvironment Revealed by Single-Cell Profiling

This study profiles single cells from multiple cancer types to create a pan-cancer blueprint of stromal cell heterogeneity, identifying shared and unique cell populations and their characteristics.

Functional heterogeneity of lymphocytic patterns in primary melanoma dissected through single-cell multiplexing.

The paper characterizes immune heterogeneity in primary melanoma using single-cell multiplex analysis, revealing functional subcategories of lymphocytic patterns and their prognostic significance.

Multiplex staining by sequential immunostaining and antibody removal on routine tissue sections

The paper describes a method for multiplex immunostaining on routine tissue sections using antibody stripping and common reagents, enabling multiple stains per section with reduced cost and complexity.