H. Ai

Promotion of RNF168-Mediated Nucleosomal H2A Ubiquitylation by Structurally-defined K63-Polyubiquitylated Linker Histone H1

This study develops a chemical synthesis method to create polyubiquitylated linker histone H1.0, revealing its role in enhancing RNF168-mediated H2A ubiquitylation through structural and biochemical analysis.

Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase

This paper develops a bioluminescent indicator for potassium ions using a modified luciferin and engineered luciferase, validated in cell lines, neurons, and live mice.

Capturing Snapshots of Nucleosomal H2A K13/K15 Ubiquitination Mediated by the Monomeric E3 Ligase RNF168

This study determines the cryo-EM structures of RNF168/UbcH5c-Ub/nucleosome complexes to elucidate the mechanism of H2A K13/K15 site-specific ubiquitination.

SHRIMP: Genetically Encoded mScarlet-derived Red Fluorescent Hydrogen Peroxide Sensor with High Brightness and Minimal Photoactivation

This paper develops SHRIMP, a genetically encoded red fluorescent hydrogen peroxide sensor with high brightness and minimal photoactivation, validated in mammalian cells and primary islet tissue.

Comprehensive catalogs for microbial genes and metagenome-assembled genomes of the swine lower respiratory tract microbiome identify the relationship of microbial species with lung lesions

The paper constructs gene catalogs and metagenome-assembled genomes from the swine lower respiratory tract microbiome and identifies associations between microbial species and lung lesions.

Structural Visualization of HECT-type E3 ligase Ufd4 Accepting and Transferring Ubiquitin to Form K29/K48-branched Polyubiquitination

This study determines the structural mechanism of HECT-type E3 ligase Ufd4 in catalyzing K29/K48-branched polyubiquitination by capturing structural snapshots of Ub transfer cascades.

Development, Characterization, and Structural Analysis of a Genetically Encoded Red Fluorescent Peroxynitrite Biosensor

The paper develops a red fluorescent peroxynitrite biosensor (pnRFP) using a genetically encoded boronic acid-containing amino acid, characterizes its properties, and elucidates its sensing mechanism through structural analysis.

Epidemiological evaluation and identification of the insect vector of soybean stay-green associated virus

This study identifies SoSGV as the causative agent of soybean stay-green syndrome and confirms the common brown leafhopper as its vector, revealing its epidemiology and geographical spread.

Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor

This paper develops a genetically encoded red fluorescent UDP-GlcNAc biosensor using a boronolectin and validates its function in live cells.

Auxin-dependent acceleration of cell division rates regulates root growth at elevated temperature

This study shows that elevated temperature promotes primary root growth in Arabidopsis thaliana by accelerating cell division rates through auxin-mediated signaling, distinct from shoot thermomorphogenesis.

An Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging in Vivo

The paper develops a red-shifted NanoLuc variant (QLuc) and uses it for in vivo immunobioluminescence imaging to improve deep-tissue visualization in mice.

Genetically encoded green fluorescent biosensors for monitoring UDP-GlcNAc in live cells

This paper introduces two genetically encoded fluorescent biosensors to monitor UDP-GlcNAc and related nucleotides in live cells, demonstrating their use in studying metabolic and pharmacological perturbations.

Structural Insights Into the Initiation and Elongation of Ubiquitination by Ubr1

This study reports cryo-EM structures of Ubr1 complexes to elucidate the mechanism of ubiquitination initiation and elongation using a single E2 enzyme.

Revisiting the evolutionary history of pigs via de novo mutation rate estimation by deep genome sequencing on a three-generation pedigree

This study estimates the de novo mutation rate in pigs using whole-genome sequencing of a three-generation pedigree and revises the evolutionary timeline of pigs, including divergence events and domestication history.

Mechanistic insight into substrate processing and allosteric inhibition of human p97

This study presents cryo-EM structures of human p97 complex with and without inhibitor NMS-873, revealing conformational changes and allosteric inhibition mechanisms.

A High-Performance Genetically Encoded Fluorescent Biosensor for Imaging Physiological Peroxynitrite

This paper introduces pnGFP-Ultra, a fluorescent biosensor for detecting peroxynitrite in live cells with high selectivity and sensitivity.

Seesaw Conformations of Npl4 in the Human p97 Complex and the Inhibitory Mechanism of a Disulfiram Derivative

This study identifies three Npl4 conformational states in the human p97 complex using cryo-EM and shows how a disulfiram derivative inhibits p97 by disrupting Npl4 zinc finger interactions.

Identification of Factors Complicating Bioluminescence Imaging

This study identifies factors affecting bioluminescence imaging results, such as ATP dependency and serum stability, to improve data interpretation.