H. Leonhardt

Microscopy analysis of delayed co-transcriptional splicing in highly expressed thyroglobulin gene

The paper uses microscopy to study delayed co-transcriptional splicing in the highly expressed thyroglobulin gene, finding a delay in splicing downstream of transcribed introns.

Generation of densely labeled oligonucleotides for the detection of small genomic elements

This paper introduces a method to generate densely labeled oligonucleotides for detecting small genomic elements using FISH in single cells.

Replisome loading reduces chromatin motion independent of DNA synthesis

This study shows that replisome loading reduces chromatin motion during S-phase, independent of DNA synthesis activity.

Altered enhancer-promoter interaction leads to MNX1 expression in pediatric acute myeloid leukemia with t(7;12)(q36;p13)

The paper shows MNX1 overexpression in t(7;12)(q36;p13) AML is due to enhancer hijacking, not oncofusion, and is common in pediatric cases.

The highly and perpetually upregulated thyroglobulin gene is a hallmark of functional thyrocytes

The paper identifies thyroglobulin gene (Tg) as a permanently upregulated gene in thyrocytes across vertebrates, providing insights into mechanisms of sustained transcriptional activation.

Comprehensive chromatin proteomics resolves functional phases of pluripotency

The paper develops a proteomic method to analyze chromatome dynamics during pluripotency phases, revealing regulatory complex binding and rearrangements.

Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger

This study determines the structure of the mouse UHRF1 PHD domain complexed with DPPA3, revealing how DPPA3 inhibits UHRF1 chromatin localization through unique interactions.

Probing protein ubiquitination in live cells

This paper introduces a fluorescent assay to detect protein ubiquitination in live cells and identifies new targets and regulators of this process.

Differences in nanoscale organization of DNase I hypersensitive and insensitive chromatin in single human cells

The paper uses microscopy and modeling to study nanoscale chromatin organization in human cells, finding cell-to-cell differences in both active and inactive regions.

Cohesin depleted cells rebuild functional nuclear compartments after endomitosis

The paper shows cohesin-depleted cells undergo endomitosis and rebuild a multilobulated nucleus with functional compartments despite lacking cohesin.

SPATIAL ORGANIZATION OF TRANSCRIBED EUKARYOTIC GENES

This study investigates how transcription shapes genome spatial organization by analyzing long highly expressed genes and demonstrating the formation of transcription loops with polymerases and nascent RNAs.

Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency

The paper compares TET1 and TET2 contributions to cytosine oxidation during naive to primed pluripotency transition, finding distinct and differentiation-dependent roles in 5hmC and 5fC formation.