N. Kobayashi

Investigating EMT-mediated resistance to EGFR tyrosine kinase inhibitors in NSCLC using innovative organoid models

This study investigates EMT-mediated resistance to EGFR TKIs in NSCLC using organoid models, finding links between EMT markers and resistance, and suggesting potential therapeutic strategies.

A human pluripotent stem cell-based somitogenesis model using microfluidics

This study develops a microfluidic hPSC-based model to control biochemical and biomechanical signals during somitogenesis, revealing their roles in somite formation.

NEDD4-binding protein 1 suppresses HBV replication by degrading HBV RNAs

This paper identifies N4BP1 as a host factor that suppresses HBV replication by degrading HBV RNAs, with its KH-like and RNase domains required for this effect.

Biophysical analysis of Gaussia Luciferase bioluminescence mechanisms using a non-oxidizable coelenterazine.

The paper investigates the bioluminescence mechanism of Gaussia Luciferase using azacoelenterazine, revealing the role of salt and monovalent anions and structural changes in catalysis.

SARS-CoV-2 causes brain inflammation via impaired neuro-immune interactions

This study develops a mouse model of SARS-CoV-2-induced brain inflammation and identifies impaired cholinergic anti-inflammatory pathway as a mechanism, suggesting donepezil as a potential treatment.

Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger

This study determines the structure of the mouse UHRF1 PHD domain complexed with DPPA3, revealing how DPPA3 inhibits UHRF1 chromatin localization through unique interactions.

Exploration of novel αβ-protein folds through de novo design

This study predicts and designs novel β-protein folds using topology rules, demonstrating their feasibility and suggesting a vast untapped space of possible folds.

Design of complicated all-α protein structures

This study designs complex all-α protein structures using helix-loop-helix motifs and canonical α-helices, achieving atomic accuracy and thermal stability in solution.

Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR

This paper determines the solution structure of Gaussia Luciferase using NMR and identifies a potential coelenterazine binding cavity involved in bioluminescence.

Erythropoietin production?by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation

This study develops a Western blot method with deglycosylation to detect Epo and ESAs in urine and plasma, showing the kidney as the main site of Epo production under hypoxia.

Orientation to polarized light in tethered flying honeybees

This study shows tethered honeybees exhibit polarotaxis by moving their abdomens in response to rotating polarized light stimuli, with preferences for specific e-vector orientations.