H. Shi

This study investigates how detergent charge affects protein orientation at the air-water interface in cryo-EM, finding that anionic detergents modify the interface charge to improve protein orientation distribution.

This paper identifies a protein-priming mechanism for SARS-CoV RNA synthesis and shows that AT-527 inhibits this process by targeting both NiRAN and RdRp domains.

The paper shows that Polycomb repressive complex 2 and Sin3 histone deacetylase complex work together to epigenetically control effector gene expression in Magnaporthe oryzae, regulating its pathogenicity.

This study develops AIM-CICs, an AI-based method using CNN to automatically identify and classify cell-in-cell structures, improving efficiency and accuracy over manual methods.

Nb15-NbH-Nb15, a bispecific nanobody with a heterotrimeric configuration, showed potent neutralization of SARS-CoV-2 in vitro and provided complete protection against infection in hACE2 mice via intranasal administration.

This study examines splenomegaly, histopathology, and splenocyte composition in mice infected with Plasmodium berghei K173, showing strain-specific differences in parasite control and splenic function.

This paper introduces delta-melt, a method using chemical modifications and melting experiments to measure thermodynamic preferences for non-native DNA conformations, revealing sequence-specific variations in conformational stability.

The paper shows m6A modification slows nucleic acid hybridization by altering conformational dynamics through isomerization pathways.

This study shows that starvation causes E. coli to shrink its inner membrane and cytoplasm, driven by water loss, and that the Tol-Pal system is essential for recovery.

This paper develops a kinetic model of DnaA regulation in E. coli to explain how cell size and cell cycle are coordinated, reproducing experimental results and predicting responses to perturbations.

This study identifies keratin 1-based epidermal green autofluorescence as a biomarker for non-invasive assessment of inflammation and oxidative stress levels in the body.

The paper presents a framework for accurate bacterial growth measurements, revealing effects of culturing conditions, initial cell density, and glycerol on growth rates and fitness.

This study identifies N4BP1 as an inhibitor of NF-κB activation by interacting with NEMO, and shows that caspase 8 cleavage inactivates N4BP1 during TRIF-dependent signaling.

The paper identifies species-specific genetic differences in ACE2 that determine SARS-CoV-2 entry capability and shows that humanizing these residues in koala and mouse ACE2 enables viral entry.

This paper develops a deep learning system for gastric cancer detection using histopathological images, achieving high sensitivity and robust performance across different scanners.

The paper shows that the interaction between UTX and DNMT1 regulates diet-induced myogenic remodeling in brown fat by maintaining brown adipocyte identity and thermogenic function.

This study shows retinoic acid reprograms enhancer landscapes in MYCN-amplified neuroblastoma cells, downregulating MYCN and inducing sympathetic differentiation.

This paper shows that recombinant Bacillus subtilis spores expressing H. contortus GAPDH protect sheep from infection by boosting immune responses and reducing parasite burden.

This study identifies NTF3 as a fat-derived neurotrophic factor that regulates sympathetic innervation and thermogenesis in adipose tissue, influencing energy metabolism and obesity.

This study shows that 2'-O-methylation stabilizes alternative RNA secondary structures and increases the lifetime of excited states in the HIV-1 TAR element.

This study investigates the effect of sodium thiocyanate (SCN-) supplementation in a DSS-induced colitis model, finding it modulates MPO activity towards HOSCN but does not prevent colitis development.

This study shows that Hoogsteen base pairs in dsDNA increase susceptibility to methylation damage, challenging the assumption that Watson-Crick faces are fully protected.

This study determines an atomic-resolution RNA ensemble for HIV-1 TAR using structure prediction and NMR refinement, revealing cooperative conformational changes.

This study examines the non-specific binding of three drug-like compounds to HIV-1 stem-loop RNAs, showing they inhibit RNA function and exhibit off-target interactions.

This paper identifies and characterizes Fnr paralogs in Paenibacillus polymyxa WLY78, showing their roles in regulating nitrogen fixation and anaerobic metabolism through gene expression changes.

This meta-analysis evaluates the prognostic role of IL-17+ cells and Th17 cells in cancer, finding high IL-17+ cells correlate with worse survival, while Th17 cells show no significant effect.

This study examines how hearing sensitivity changes with age in red-eared slider turtles, finding frequency-specific developmental patterns and age-related shifts in auditory thresholds.

This paper shows that increasing cryo-EM sample freezing temperature reduces beam-induced motion and improves reconstruction resolution by including more undamaged initial frames.

The paper quantifies surface area to volume ratio dynamics in bacteria under environmental changes and develops a model linking these dynamics to gene expression and growth control.

The paper quantifies how translation inhibition reduces transcription capacity by limiting free RNA polymerase availability, affecting gene expression based on mRNA length.