J. M. Kollman

Structural basis for allosteric regulation of human phosphofructokinase-1

This study determines the cryoEM structures of human PFK1 in R- and T-states, revealing eukaryotic allosteric regulation mechanisms and the role of the C-terminus in autoinhibition.

A family of bacterial actin homologues forms a 3-stranded tubular structure

The paper identifies a family of bacterial actins forming a unique 3-stranded tubular structure via ATP binding, revealing new insights into bacterial cytoskeletal diversity.

Light-sensitive phosphorylation regulates enzyme activity and filament assembly of human IMPDH1 retinal splice variants

This paper shows phosphorylation at S477 regulates IMPDH1 filament assembly and activity, modulating GTP synthesis in retinal cells.

Toxoplasma gondii actin filaments are tuned for rapid disassembly and turnover

This study investigates the dynamic properties of Toxoplasma gondii actin filaments, showing they undergo rapid disassembly and turnover due to unique biochemical features.

Blueprinting expandable nanomaterials with standardized protein building blocks

This paper introduces standardized protein building blocks and geometric design principles to create expandable protein nanomaterials, validated through X-ray crystallography and electron microscopy.

Point mutations in IMPDH2 which cause early-onset neurodevelopmental disorders disrupt enzyme regulation and filament structure

The paper identifies two new IMPDH2 mutations linked to neurodevelopmental disorders and shows they disrupt GTP regulation and enzyme function through structural and functional analysis.

Human PRPS1 filaments stabilize allosteric sites to regulate activity

This study determines structures of PRPS1 filaments in active and inhibited states, revealing how filament stability regulates enzyme activity and links to disease.

Cryo-EM Structures of CTP Synthase Filaments Reveal Mechanism of pH-Sensitive Assembly During Budding Yeast Starvation

The paper uses cryo-EM to study CTP synthase filament assembly in yeast, revealing pH-sensitive mechanisms and their role in starvation response.

IMPDH1 retinal variants control filament architecture to tune allosteric regulation

The paper shows IMPDH1 retinal variants form different filaments that affect GTP regulation, with disease mutations disrupting or not affecting this regulation.

The Giardia lamellipodium-like ventrolateral flange supports attachment and rapid cytokinesis

This paper identifies Flangin, an actin-associated protein essential for forming the ventrolateral flange (VLF) in Giardia, which supports attachment and rapid cytokinesis.

Coupled structural transitions enable highly cooperative regulation of human CTPS2 filaments

The paper reveals how human CTPS2 filaments use coupled structural transitions to enable highly cooperative regulation through dynamic switching between active and inactive states.

Independent evolution of polymerization in the Actin ATPase clan regulates hexokinase activity

Yeast glucokinase activity is limited by its polymerization, which is critical for cell viability during glucose refeeding.

The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane

This study identifies the Mla system in Acinetobacter baumannii as essential for glycerophospholipid transport to the outer membrane and antibiotic resistance.

IMPDH polymers accommodate both catalytically active and inactive conformations

The paper investigates IMPDH polymerization and finds that polymerized and non-assembled forms have comparable catalytic activity, with substrate and nucleotide availability affecting filament conformation.

Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids.

This paper describes a method to improve particle density on cryo-EM grids through multiple rounds of sample application and blotting, enhancing structural studies of various biomolecules.