Joseph Collin

Single-Cell and Tissue-Specific CRISPR Editing Analyses Unveil New Insights to Off-Targets and Translocations

The paper analyzes CRISPR-Cas9 off-target mutations and translocations at single-cell and tissue-specific levels in mice, revealing context-dependent variability in editing outcomes.

Deciphering the spatio-temporal transcriptional and chromatin accessibility of human retinal organoid development at the single cell level

This study presents the first single-cell spatio-temporal transcriptome of human retinal organoid development using single-cell RNA-Seq and spatial transcriptomics, revealing key developmental events and chromatin accessibility patterns.

Transplanted pluripotent stem cell-derived photoreceptor precursors elicit conventional and unusual light responses in mice with advanced retinal degeneration

This study shows that transplanted pluripotent stem cell-derived cone precursors can restore light responses in mice with advanced retinal degeneration, despite low engraftment.

A single cell atlas of human cornea that defines its development, limbal stem and progenitor cells and the interactions with the limbal niche

This paper generates a single-cell atlas of the human cornea across development and disease, identifying key cell types, markers, and interactions that inform stem cell maintenance and disease mechanisms.

Human iPSC-derived RPE and retinal organoids reveal impaired alternative splicing of genes involved in pre-mRNA splicing in PRPF31 autosomal dominant retinitis pigmentosa

The paper generates patient-specific retinal organoids and RPE from RP type 11 patients to show PRPF31 mutations cause retinal-specific splicing defects and structural/functional impairments, which are corrected by in situ gene editing.