M. C. Skala

Label free metabolic imaging to enhance the efficacy of Chimeric Antigen Receptor T cell therapy

Autofluorescence imaging informs manufacturing conditions that enhance yield and metabolic fitness of CAR T cells for neuroblastoma.

Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence

This study shows that metabolic priming during CAR T cell manufacturing reduces glycolysis and enhances memory phenotypes, improving in vivo persistence against GD2+ tumors.

Quantifying in vivo collagen reorganization during immunotherapy in murine melanoma with second harmonic generation imaging

This study quantifies collagen reorganization in murine melanoma during immunotherapy using second harmonic generation imaging, finding treatment-induced changes in collagen fiber morphology and density.

Naive primary neutrophils play a dual role in the tumor microenvironment

This study investigates the dual role of naive primary neutrophils in the tumor microenvironment, showing they both restrict tumor invasion and hinder NK cell penetration through ROS and NETs.

Metabolic changes to host cells with Toxoplasma gondii infection

This study examines how Toxoplasma gondii infection alters host cell metabolism using multiple techniques, finding similar metabolic changes during infection and the pre-invasion 'kiss and spit' process.

Optical coherence tomography of human fetal membrane sub-layers during dynamic loading

This study uses optical coherence tomography to analyze the mechanical properties of human fetal membranes under dynamic loading, identifying the amnion as the load-bearing layer and location-dependent thickness variations.

cell-analysis-tools: an open-source library for single-cell analysis of multi-dimensional microscopy images

The paper introduces an open-source library to streamline single-cell analysis of multi-dimensional microscopy images, providing tools for quality checking, automation, and visualization of trends.

Light sheet autofluorescence lifetime imaging with a single photon avalanche diode array

This paper introduces a light sheet FLIM system using SPAD arrays for fast, label-free single-cell NAD(P)H autofluorescence imaging in live cells and organisms.

Autofluorescence lifetime imaging classifies human lymphocyte activation and subtype

This paper uses optical metabolic imaging to classify human lymphocyte activation and subtype with high accuracy using autofluorescence lifetime parameters.

Differential metabolic adaptations define responses of winner and loser oncogenic mutant stem cells in skin epidermis in vivo

The paper investigates metabolic adaptations in winner and loser oncogenic mutant stem cells in skin epidermis, revealing distinct metabolic strategies that drive cell competition outcomes.

Inflammatory CD4/CD8 double positive human T cells arise from reactive CD8 T cells and are sufficient to mediate GVHD pathology

This study identifies CD4+/CD8+ double positive T cells as mediators of xenogeneic GVHD but with limited GVL activity.

Label-free imaging to track reprogramming of human somatic cells

This study uses label-free live-cell imaging and machine learning to track reprogramming of human somatic cells and identify high-quality iPSCs with ~95% accuracy.

Integrating Subclonal Response Heterogeneity to Define Cancer Organoid Therapeutic Sensitivity

The paper evaluates individual patient-derived cancer organoids using optical metabolic imaging to predict therapeutic response and identify subclonal populations and resistance mechanisms.

Impact of baseline culture conditions of mouse-derived cancer organoids when determining therapeutic response and tumor heterogeneity

This study evaluates how baseline culture conditions affect therapeutic response and heterogeneity in mouse-derived cancer organoids, finding that initial size is the key factor influencing growth and response.

Patient-derived cancer organoid tracking with widefield one-photon redox imaging to assess treatment response

This study uses redox imaging to track individual cancer organoids and assess treatment response by measuring metabolic and morphological changes.

Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors

The paper investigates how interactions with pancreatic stromal cells affect the redox state of cancer cells, finding that these interactions promote a more oxidized redox state in cancer cells.

Adaptable pulsatile flow generated by quantitative imaging of stem-cell derived cardiomyocytes for disease modeling

This study introduces an adaptable pump system to generate pulsatile flows from cardiac spheroids, enabling modeling of endothelial cell behavior under different mechanical stresses.

Graphene nanoflakes for acute manipulation of membrane cholesterol and transmembrane signaling

This study shows graphene nanoflakes manipulate membrane cholesterol, altering cell signaling in fibroblasts and neurons.

Optical Metabolic Imaging of Heterogeneous Drug Response in Pancreatic Cancer Patient Organoids

This study uses optical metabolic imaging to predict pancreatic cancer patient treatment response by analyzing cellular heterogeneity in organoids.

Label-free Method for Classification of T cell Activation

This paper develops a label-free method using NAD(P)H and FAD autofluorescence imaging and machine learning to classify T cell activation states and subtypes with high accuracy.