P. C. Blainey

Single-cell image-based genetic screens systematically identify regulators of Ebola virus subcellular infection dynamics

This study uses single-cell image-based CRISPR screens to identify host factors regulating Ebola virus infection, revealing new therapeutic targets like UQCRB and STRAP.

Massively parallel combination screen reveals small molecule sensitization of antibiotic-resistant Gram-negative ESKAPE pathogens

This study uses high-throughput screening to identify small molecules that enhance antibiotic efficacy against multidrug-resistant Gram-negative bacteria.

De novo mutations mediate phenotypic switching in an opportunistic human lung pathogen

This study shows that de novo mutations in Burkholderia dolosa cause phenotypic switching, with O-antigen loss increasing immune cell uptake but reducing lung competitiveness, depending on infection duration and tissue localization.

Vaginal Lactobacillus fatty acid response mechanisms reveal a novel strategy for bacterial vaginosis treatment

This study identifies oleic acid as a compound that inhibits L. iners and promotes L. crispatus growth, offering a potential new treatment strategy for bacterial vaginosis.

KatG catalase deficiency confers bedaquiline hyper-susceptibility to isoniazid resistant Mycobacterium tuberculosis

This study identifies KatG catalase deficiency in INH-resistant M. tuberculosis as a factor contributing to hyper-susceptibility to bedaquiline.

Multiplexed detection of bacterial nucleic acids using Cas13 in droplet microarrays

This paper introduces bCARMEN, a high-throughput system for rapid, multiplexed bacterial pathogen detection and antibiotic resistance gene identification using CRISPR-Cas13 and droplet microfluidics.

Induction of a regulatory myeloid program in bacterial sepsis and severe COVID-19

The paper identifies a regulatory myeloid program, MS1, associated with sepsis mortality and up-regulated in severe COVID-19, showing its immunosuppressive role and cytokine-dependent induction.

Consensus transcriptional states describe human mononuclear phagocyte diversity in the lung across health and disease

The paper identifies conserved transcriptional programs in lung mononuclear phagocytes across diseases using a unified framework and applies it to characterize disease-associated populations.

Hydrogenotrophic methanogenesis in archaeal phylum Verstraetearchaeota reveals the shared ancestry of all methanogens

The paper discovers a hydrogenotrophic methanogenesis pathway in Verstraetearchaeota and the Wood-Ljungdahl pathway in Desulfurococcales, suggesting an ancient origin of hydrogenotrophic methanogenesis and a later adaptation to methylotrophic methanogenesis.

Multiplexed enrichment and genomic profiling of peripheral immune cell subsets on a microfluidic chip

This paper presents a microfluidic system for sorting and profiling immune cell subsets using RNA-seq, demonstrating its utility in identifying cell-specific disease signatures in lupus patients.

Combinatorial Drug Discovery in Nanoliter Droplets

This paper develops a high-throughput screening system for discovering synergistic drug combinations and applies it to identify new antibiotic synergies against E. coli.

Targeting individual cells by barcode in pooled sequence libraries

The paper introduces a method combining molecular enrichment with FACS to efficiently sample rare dendritic cell populations in single-cell RNA-Seq libraries.

Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples

This paper introduces a microfluidic-based mini-metagenomic method to extract novel microbial genomes from complex environmental samples, revealing 29 new genomes with diverse metabolic pathways and SNP distributions.