J. Broichhagen

Deuteration provides a general strategy to enhance azobenzene-based photopharmacology

This paper introduces deuterated azobenzene photoswitches to enhance photopharmacological molecules by improving light sensitivity, photoswitch efficiency, and kinetics.

A neuronal circuit driven by GLP-1 in the olfactory bulb regulates insulin secretion

This study shows that GLP-1 receptor activation in the olfactory bulb enhances insulin secretion via a sympathetic pathway involving the paraventricular nucleus.

Direct optical measurement of intra-molecular distances down to the Angstrom scale

This paper demonstrates that MINFLUX fluorescence nanoscopy can measure intra-molecular distances down to 1 Angstrom with high precision, validated using known protein distances and subunit orientations.

An examination of the divergent spatiotemporal signaling of GLP-1R versus GIPR in pancreatic beta cells

This study compares the trafficking and signaling of GLP-1R and GIPR in pancreatic beta cells, revealing differences in internalization, recycling, and subcellular activity.

14-3-3ζ constrains insulin secretion by regulating mitochondrial function in pancreatic β-cells

This study shows that 14-3-3ζ regulates insulin secretion in pancreatic β-cells by enhancing mitochondrial function and ATP synthesis, with implications for diabetes.

A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

The paper introduces a Halo- and SNAP-tagged BMP/TGFβ receptor library and establishes a Ligand Surface Binding Assay to quantify cell surface ligand binding specificity.

Sequential in vivo labeling of insulin secretory granule pools in INS-SNAP transgenic pigs

This study develops a transgenic pig model with SNAP-tagged insulin to investigate in vivo insulin secretory granule dynamics and turnover.

Evaluation of efficacy- versus affinity-driven agonism with biased GLP-1R ligands P5 and exendin-F1

This study compares the efficacy and affinity of two GLP-1R agonists, P5 and exendin-F1, showing that exendin-F1's lower acute efficacy correlates with sustained insulin secretion and anti-hyperglycaemic effects.

Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking

This study compares exendin-4 and its acylated variant exendin-4-C16, showing acylation affects GLP-1R signaling bias, trafficking, and pharmacokinetics.

Sulfonated rhodamines as impermeable labelling substrates for cell surface protein visualization

The paper introduces sulfonated rhodamines as impermeable dyes for cell surface protein labeling, validated in live cell imaging and STED nanoscopy.

Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin

The paper investigates the dimerization behavior of F220C rhodopsin mutant and wild-type opsin in vitro and in the plasma membrane, finding that the mutant forms a unique dimer with distinct conformational features.

Deuterated rhodamines for protein labelling in nanoscopy

This paper introduces deuterated rhodamines for improved fluorescence microscopy applications, showing enhanced photophysical and chemical properties.

Purification of age-distinct insulin secretory granules through antigen restriction

The paper purifies age-distinct insulin secretory granules from INS-1 cells and identifies age-related changes in their lipid composition and membrane properties.

The influence of peptide context on signalling and trafficking of glucagon-like peptide-1 receptor biased agonists

The paper evaluates how peptide context affects GLP-1R signaling and trafficking, showing differences in agonist-mediated responses based on sequence homology and structural features.

Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates

The paper introduces SBG-conjugated fluorophores to improve extracellular labeling of SNAP-fused proteins, enabling precise analysis of GPCR populations in living cells and tissues.

Differences in signalling, trafficking and glucoregulatory properties of glucagon-like peptide-1 receptor agonists exendin-4 and lixisenatide

This study compares the signalling, trafficking, and glucoregulatory effects of exendin-4 and lixisenatide, finding differences in cAMP signalling potency and receptor recycling that impact glucose control.

LUXendins reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

The paper introduces LUXendin peptides that label and visualize endogenous GLP1R with high specificity, revealing receptor organization and dynamics.

Branched photoswitchable tethered ligands enable ultra-efficient optical control and detection of class C G protein-coupled receptors

This paper develops branched photoswitchable ligands for efficient optical control and detection of class C G protein-coupled receptors, demonstrating near-complete agonism and dual imaging/manipulation capabilities.

SNAP-tagged nanobodies enable reversible optical control of a G protein-coupled receptor via a remotely tethered photoswitchable ligand

This paper introduces a method to reversibly control GPCR activity using photoswitchable ligands tethered via SNAP-tagged nanobodies.