S. R. Manalis

Phenotypic screens identify biologic regulators of nanoparticle uptake in diffuse midline glioma

The paper identifies biologic regulators of nanoparticle uptake in diffuse midline glioma using CRISPR screens and functional assays, finding CTNNB1 and pathway members as key modulators.

Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth

The paper isolates two novel marine cyanobacteria from CO2-rich environments, characterizes their rapid growth and high biomass density, and suggests their potential for CO2-based bioproduction.

Leukemia circulation kinetics revealed through blood exchange method

This study uses a blood exchange method in mouse leukemia models to show that leukemia cells circulate longer than solid tumor cells and that microenvironmental factors like E-selectin influence their clearance.

Direct quantification of unicellular algae sinking velocities reveals cell size, light, and nutrient-dependence

The paper quantifies sinking velocities of unicellular algae to show their dependence on cell size, light, and nutrients, revealing mechanisms linking cell composition to vertical movement.

Single-cell mass distributions reveal simple rules for achieving steady-state growth

The paper shows that steady-state growth in bacterial cultures occurs only after high dilution from stationary phase and is characterized by stable mass distribution.

Single-cell monitoring of dry mass and dry density reveals exocytosis of cellular dry contents in mitosis

The paper develops a single-cell method to monitor dry mass and density during mitosis, revealing exocytosis-driven changes in cellular composition.

Nucleotide imbalance decouples cell growth from cell proliferation

The paper shows that nucleotide imbalances during S phase disrupt DNA replication and cell proliferation, requiring replication stress signaling for survival.

Weighing the DNA content of Adeno-Associated Virus vectors with zeptogram precision using nanomechanical resonators

This paper introduces a method using nanomechanical resonators to measure AAV vector mass with high precision, enabling real-time quality control in biomanufacturing.

The tumor microenvironment drives transcriptional phenotypes and their plasticity in metastatic pancreatic cancer

This study identifies a transitional cancer cell state in metastatic pancreatic cancer and shows that tumor microenvironment signals drive transcriptional plasticity and subtype transitions.

Functional drug susceptibility testing based on biophysical measurements predicts patient outcome in glioblastoma patient-derived neurosphere models

Using biophysical assays on glioblastoma patient-derived neurosphere models, the study finds that ex vivo drug susceptibility testing predicts patient survival with similar accuracy to MGMT promoter methylation.

HSP90 facilitates oncogenic alterations of metabolism in B-cell lymphomas

This study shows HSP90 supports metabolic compartmentalization in B-cell lymphomas, and its inhibition reduces cancer metabolism.

Mass measurements of polyploid lymphocytes reveal that growth is not size limited but depends strongly on cell cycle

The paper measures mass growth of polyploid mouse lymphocytes to show that cell cycle, not cell size, primarily determines growth efficiency.

Time-dynamics of mitochondrial membrane potential reveal an inhibition of ATP synthesis in mitosis

The paper quantifies mitochondrial ATP synthesis rates during mitosis and finds a 50% decrease in early mitosis linked to CDK1 activity.

Linking single-cell measurements of mass, growth rate, and gene expression

This paper introduces a microfluidic platform that links single-cell biophysical measurements with scRNA-seq to study gene expression related to cell mass and growth rate.