Author

T. M. Kapoor

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Biophysics· 3 Cancer Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 8 Organism:human· 4 Research Focus:drug response / drug discovery· 2 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1 Assay:cryo-EM· 1 Research Focus:host-pathogen & immune response· 1 Assay:imaging· 1

Frequent Co-Authors

B. T. Chait· 4 shared L. Urnavicius· 4 shared K. J. Mickolajczyk· 3 shared A. Aher· 2 shared K. R. Molloy· 2 shared L. E. Vostal· 2 shared M. Grasso· 2 shared M. Wieczorek· 2 shared

Activity by Year

2019-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 28 mutual co-author links

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4 shared papersBiochemistry

B. T. Chait

Mutual co-authors in cluster

Author profile →

9 papers

Recent·Most cited

Allosteric activation of VCP, a AAA unfoldase, by small molecule mimicry

N. H. Jones, Q. Liu, L. Urnavicius, N. E. Dahan, L. E. Vostal, T. M. Kapoor

This paper identifies VA1, a small molecule that activates VCP/p97, and reveals its allosteric mechanism through cryo-EM structures and mutagenesis studies.

bioRxiv · Oct 2, 2023 · PreprintBiochemistry

Using a function-first "scout fragment"-based approach to develop allosteric covalent inhibitors of conformationally dynamic helicase mechanoenzymes

J. R. Ramsey, P. M. M. Shelton, T. K. Heiss, P. D. B. Olinares, L. E. Vostal, H. Soileau, M. Grasso, S. Warrington, S. Adaniya, M. Miller, S. Sun, D. J. Huggins, R. W. Myers, B. T. Chait, E. V. Vinogradova, T. M. Kapoor

The paper develops covalent inhibitors for conformationally dynamic helicases using electrophilic scout fragments and a function-first approach, targeting SARS-CoV-2 nsp13 and human helicases BLM and WRN.

bioRxiv · Sep 25, 2023Biochemistry

High-content microscopy reveals a morphological signature of bortezomib resistance

M. E. Kelley, A. Y. Berman, D. R. Stirling, B. A. Cimini, Y. Han, S. Singh, A. E. Carpenter, T. M. Kapoor, G. P. Way

The paper identifies a morphological signature of bortezomib resistance using high-content microscopy and shows it predicts treatment response in independent cell lines.

bioRxiv · May 2, 2023Cancer Biology

Inhibition of the SARS-CoV-2 helicase at single-nucleotide resolution.

S. K. Marx, K. J. Mickolajczyk, J. M. Craig, C. A. Thomas, A. M. Pfeffer, S. J. Abell, J. D. Carrasco, M. C. Franzi, J. R. Huang, H. C. Kim, H. D. Brinkerhoff, T. M. Kapoor, J. H. Gundlach, A. H. Laszlo

This study uses nanopore tweezers to analyze SARS-CoV-2 helicase nsp13 motion and inhibition at single-nucleotide resolution, revealing kinetic mechanisms and inhibitor effects.

bioRxiv · Oct 7, 2022 · PreprintBiophysics

The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrates

K. J. Mickolajczyk, P. D. B. Olinares, B. T. Chait, S. Liu, T. M. Kapoor

This study investigates how the MIDAS domain of Mdn1 forms catch bonds with two different UBL substrates under force, revealing force-dependent binding lifetime changes.

bioRxiv · Aug 30, 2021Biophysics

Targeting Allostery in the Dynein Motor Domain with Small Molecule Inhibitors

C. C. Santarossa, K. Mickolajczyk, J. B. Steinman, L. Urnavicius, N. Chen, Y. Fukase, Y. Hirata, N. Coudray, D. C. Ekiert, G. Bhabha, T. M. Kapoor

This study develops small molecule inhibitors targeting allostery in dynein motor domains by inhibiting regulatory ATPase sites rather than the main catalytic site.

bioRxiv · Sep 22, 2020Biochemistry

Biochemical reconstitutions reveal principles of human γ-TuRC assembly and function

M. Wieczorek, S.-C. Ti, L. Urnavicius, K. R. Molloy, A. Aher, B. T. Chait, T. M. Kapoor

This paper reconstitutes the human γ-TuRC and analyzes its structure and function, showing that the lumenal bridge is essential for microtubule nucleation.

bioRxiv · Sep 21, 2020Biochemistry

Force-dependent stimulation of RNA unwinding by SARS-CoV-2 nsp13 helicase

K. J. Mickolajczyk, P. M. M. Shelton, M. Grasso, X. Cao, S. R. Warrington, A. Aher, S. Liu, T. M. Kapoor

This study investigates the force-dependent unwinding activity of SARS-CoV-2 nsp13 helicase using optical tweezers, revealing increased unwinding frequency, processivity, and velocity under destabilizing force.

bioRxiv · Jul 31, 2020Biophysics

Asymmetric molecular architecture of the human γ-tubulin ring complex

M. Wieczorek, L. Urnavicius, S.-C. Ti, K. R. Molloy, B. T. Chait, T. M. Kapoor

This study presents a cryo-EM structure of the native human γ-tubulin ring complex, revealing its asymmetric architecture and identifying new structural features and interactions.

bioRxiv · Oct 25, 2019Biochemistry