V. Gradinaru

Human cell surface-AAV interactomes identify LRP6 as blood-brain-barrier transcytosis receptor and immune cytokine IL3 as AAV9 binder

Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo

The paper identifies transcriptional crosstalk between enhancers and promoters delivered by AAVs, showing concatemer formation enables targeted delivery of large genetic cargo for cell-type-specific gene editing.

Primate-conserved Carbonic Anhydrase IV and murine-restricted Ly6c1 are new targets for crossing the blood-brain barrier

The paper identifies two new receptors, Ly6c1 and Car4/CA4, for crossing the blood-brain barrier using AAV vectors and computational modeling.

Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates

The paper develops AAV vectors with endothelial-specific tropism for brain delivery in rodents and broad tropism in primates, demonstrating their utility in genetic engineering of the blood-brain barrier.

Fast, accurate ranking of engineered proteins by receptor binding propensity using structure modeling

This paper introduces APPRAISE, a method that rapidly ranks engineered proteins by their receptor binding propensity using structure modeling, demonstrating its accuracy on various protein classes.

A prebiotic diet modulates microglial states and motor deficits in α-synuclein overexpressing mice

A prebiotic diet reduces motor deficits and microglial activation in α-synuclein overexpressing mice by altering gut microbiota and microglial states.

Intravenous gene transfer throughout the brain of infant Old World primates using AAV

This paper introduces AAV.CAP-Mac, an engineered AAV variant that enables efficient systemic brain-wide gene delivery in infant Old World primates.

Multi-Wavelength Analytical Ultracentrifugation of Biopolymer Mixtures and Interactions

The paper demonstrates the utility of multi-wavelength analytical ultracentrifugation for analyzing complex biopolymer mixtures and interactions.

Deep parallel characterization of AAV tropism and AAV-mediated transcriptional changes via single-cell RNA sequencing

The paper develops a scRNA-seq pipeline to characterize AAV tropism and transcriptional responses at single-cell resolution, revealing cell-type-specific targeting and gene expression changes.

Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology

This study activates specific gut neurons in mice to show they influence microbiome structure and gut physiology, including bile acids and fecal output, without brain signals.

Broad gene expression throughout the mouse and marmoset brain after intravenous delivery of engineered AAV capsids

The paper identifies AAV capsid variants that target the brain with high specificity and avoid liver expression in mice and marmoset non-human primates.

Machine learning-guided channelrhodopsin engineering enables minimally-invasive optogenetics

The paper engineers light-gated channelrhodopsins with high photocurrent and light sensitivity, enabling minimally-invasive optogenetics without invasive surgery or implants.