Jeremy E. Wilusz

This paper develops a system to produce high-purity circular RNAs by minimizing linear RNA byproducts through optimized circularization and transcript termination strategies.

The paper introduces LIDAR, a ligation-independent sequencing method that captures diverse RNA species, including tRNA-derived RNAs with blocked 3' termini, in mouse cells.

The paper shows that IntS6 recruits PP2A to regulate transcription termination efficiency at specific genes, highlighting the phosphatase module's selective role in Integrator complex function.

The paper introduces a toolkit for efficiently and scarlessly overexpressing circular RNAs by redesigning plasmid vectors to eliminate molecular scars at back-splice junctions.

The paper shows that Integrator complex depletion disrupts histone gene expression timing by dispersing transcription machinery clusters.

The paper identifies cell-type-specific transcription factors that bind rDNA and regulate rRNA transcription, showing CEBPA's role in promoting Pol I occupancy and rRNA synthesis in myeloid progenitors.

The paper investigates whether circular or linear RNA topology affects Target RNA-Directed MicroRNA Degradation (TDMD), finding that circular RNAs can inhibit while linear forms can drive TDMD.

The paper compares off-target effects of CRISPR/Cas13 effectors in eukaryotic cells, finding significant variability in specificity across different effectors and cell types.

The paper shows the Integrator complex destabilizes paused RNA polymerase II at promoters, leading to termination of non-productive transcription.

The paper identifies the Integrator complex as a regulator of transcription by cleaving nascent mRNAs, revealing its role in attenuating gene expression during copper stress.