This study generates hypoimmunogenic gastric insulin-secreting organoids (GINS) and shows PD-L1 engineering improves their survival under immune challenge.

bioRxiv · Jul 13, 2026 · PreprintImmunology

Niche-driven phenotypic plasticity and cis-regulatory dynamics of a revised model for intestinal secretory differentiation

Swarnabh Bhattacharya, Guodong Tie, Pratik Narendra Pratap Singh, Ermanno Malagola, Onur Eskiocak, Ruiyang He, Judith Kraiczy, Wei Gu, Yakov Perlov, Aybuke Alici-Garipcan, Semir Beyaz, Timothy Cragin Wang, Qiao Zhou, Ramesh A. Shivdasani

The paper shows that goblet and Paneth cells share similar gene expression and chromatin features, and their phenotypes are influenced by niche signals like BMP, suggesting a single cell type with plasticity.

Cell stem cell · Feb 8, 2025Peer-reviewedCell Biology

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation

Pratik Narendra Pratap Singh, Wei Gu, Shariq Madha, Allen W. Lynch, Paloma Cejas, Ruiyang He, Swarnabh Bhattacharya, Miguel Muñoz Gomez, Matthew G. Oser, Myles A Brown, Henry W. Long, Clifford A. Meyer, Qiao Zhou, Ramesh A. Shivdasani

This study investigates transcription factor dynamics during human enteroendocrine cell differentiation, finding that oscillations of ASCL1 and NEUROD1 enable accurate cell type diversification.

Cell stem cell · May 10, 2024Peer-reviewedMolecular Biology

A MTA2-SATB2 chromatin complex restrains colonic plasticity toward small intestine by retaining HNF4A at colonic chromatin

Wei Gu, Xiaofeng Huang, Pratik Narendra Pratap Singh, Sanlan Li, Ying Lan, Min Deng, Lauretta A. Lacko, Jesús M. Gómez-Salinero, Shahin Rafii, Michael Paul Verzi, Ramesh A. Shivdasani, Qiao Zhou

The paper identifies MTA2 and SATB2 as a chromatin complex that maintains colonic identity by retaining HNF4A on colonic chromatin, preventing plasticity toward small intestine.

Nature Communications · Apr 27, 2024Peer-reviewedDevelopmental Biology