Aaron C. Goldstrohm

Global Regulatory Functions of the Human CCR4-NOT Complex in mRNA Deadenylation and Decay

The paper identifies the regulatory roles of the CCR4-NOT complex in mRNA deadenylation and decay by analyzing transcriptome-wide effects of subunit depletion.

RNA-binding is the essential biological function of the Drosophila protein Brat

This study shows that RNA-binding is essential for Brat function in Drosophila development by generating and testing RNA-binding defective mutant alleles.

Human Pumilio proteins use fuzzy multivalent hydrophobic interactions to recruit the CCR4-NOT deadenylase complex to repress mRNAs

The paper identifies how human PUM proteins repress mRNAs by using fuzzy multivalent hydrophobic interactions to recruit the CCR4-NOT deadenylase complex through their intrinsically disordered RD3 domain.

Cytoplasmic poly-adenosine binding proteins modulate susceptibility of mRNAs to RNA-binding protein-directed decay

This study shows that PABPCs modulate PUM1/2-mediated mRNA decay by protecting poly(A) tails, with PABPC levels determining mRNA susceptibility to degradation.

Chemi-Northern: a versatile chemiluminescent northern blot method for analysis and quantitation of RNA molecules.

The paper introduces Chemi-Northern, a chemiluminescent northern blot method for RNA detection and quantitation, showing it is sensitive, fast, and suitable for analyzing natural and engineered RNAs.

Regulation of the Drosophila transcriptome by Pumilio and CCR4-NOT deadenylase

This study identifies novel Pumilio target genes and reveals how Pumilio and CCR4-NOT deadenylase regulate mRNA stability and translation efficiency in Drosophila.

Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptome

This study identifies how PUM1 and PUM2 regulate gene expression by interacting with the CCR4-NOT complex and DCP2 to repress mRNA decay.

Differential processing and localization of human Nocturnin controls metabolism of mRNA and nicotinamide dinucleotide metabolites

This study shows that human Nocturnin regulates mRNA levels and NADP(H) cofactors based on its subcellular localization.

Principles of mRNA control by human PUM proteins elucidated from multi-modal experiments and integrative data analysis

The paper identifies how human PUM proteins regulate gene expression by RNA stability and develops a rulebook to predict functional PREs using machine learning.

Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs.

This study identifies that Pumilio's repression domains use deadenylation and decapping factors to accelerate mRNA degradation in Drosophila.