The paper identifies LEVER RNA as a regulator of ε-globin expression by inhibiting PRC2-mediated H3K27 methylation and disrupting promoter-LCR interactions.

Communications Biology · Apr 11, 2022Peer-reviewedMolecular Biology

Variant Library Annotation Tool (VaLiAnT): an oligonucleotide library design and annotation tool for Saturation Genome Editing and other Deep Mutational Scanning experiments

Luca Barbon, Victoria Offord, Elizabeth J. Radford, Adam P. Butler, Sebastian S. Gerety, David J. Adams, Matthew E. Hurles, Hong Kee Tan, Andrew J. Waters

Bioinformatics · Nov 10, 2021Peer-reviewedGenomics

Targeted intragenic demethylation initiates chromatin rewiring for gene activation

Yanjing V. Liu, Mahmoud Adel Bassal, Migara Kavishka Jayasinghe, Quy Xiao Xuan Lin, Chan-Shuo Wu, Jing Ping Tang, Junsu Kwon, Qiling Zhou, Hong Kee Tan, Alexander K. Ebralidze, Minh T. N. Le, Li Chai, Touati Benoukraf, Annalisa Di Ruscio, Daniel Geoffrey Tenen

CRISPR-DiR induces locus-specific demethylation and chromatin remodeling to reactivate the p16 gene.

bioRxiv · Jul 16, 2020 · PreprintGenetics

Zinc finger protein SALL4 functions through an AT-rich motif to regulate gene expression

Nikki R. Kong, Mahmoud Adel Bassal, Hong Kee Tan, Jesse V. Kurland, Kol Jia Yong, John J. Young, Yang Yang, Fudong Li, Jonathan D. Lee, Yue Liu, Chan-Shuo Wu, Alicia J. Stein, Hongbo Robert Luo, Leslie E. Silberstein, Martha L. Bulyk, , Li Chai

The paper identifies the DNA binding domain and sequence of SALL4, discovers its target genes in liver cancer cells, and reveals its mechanism of gene regulation.

Cell Reports · Jan 1, 2020Peer-reviewedMolecular Biology

Widespread interaction between ADAR1 and transcriptional byproducts

Chan-Shuo Wu, Sze Jing Tang, Hong Kee Tan, Li-Yuan Hung, Wei Wen Teo, Jia Li, Ömer An, Kar Tong Tan, Qiling Zhou, Leilei Chen, Daniel Geoffrey Tenen, Henry He Yang

This study identifies ADAR1's interaction with transcriptional byproducts using integrated sequencing data, revealing its role in RNA processing and debris clearance.

bioRxiv · Dec 13, 2019 · PreprintGenomics