Author

Annalisa Di Ruscio

Università degli Studi del Piemonte Orientale “Amedeo Avogadro”ORCID 0000-0002-9705-4245Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 4 Genetics· 2 Neuroscience· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Assay:DNA methylation· 4 Organism:human· 4 Research Focus:drug response / drug discovery· 3 Research Focus:gene regulatory network inference· 3 Research Focus:differential expression· 2 Research Focus:cell type annotation· 1 Assay:long-read sequencing· 1

Frequent Co-Authors

Alexander K. Ebralidze· 6 shared Mahmoud Adel BassaliD· 6 shared Simone UmmarinoiD· 6 shared Daniel Geoffrey TeneniD· 4 shared Bon Q. TrinhiD· 3 shared Giulia GaggiiD· 3 shared Lucrezia RinaldiiD· 3 shared Marta BorchielliniiD· 3 shared

Activity by Year

2019-2025 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 44 mutual co-author links

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6 shared papersMolecular Biology

Alexander K. Ebralidze

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

Small activating RNA AW1-51 (CEBPA-51) elicits targeted DNA demethylation to promote gene activation

Giulia Gaggi, Juan Rodrigo Patiño-Mercau, Marta Borchiellini, Mailin Li, Lucrezia Rinaldi, Giorgia Maroni, Davide D’Onghia, Susumu S. Kobayashi, Mahmoud Adel Bassal, Nagy Adley Habib, Pedro P. Medina, Angela Di Baldassarre, Barbara Ghinassi, Alexander K. Ebralidze, Simone Ummarino, , Annalisa Di Ruscio

This study identifies the mechanism by which AW1-51, a small activating RNA, promotes DNA demethylation and reactivation of the CEBPA gene in cancer.

bioRxiv · Sep 29, 2025 · PreprintMolecular Biology

Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation

Carla Lucia Esposito, Ida Autiero, Annamaria Sandomenico, Haiqing Li, Mahmoud Adel Bassal, Maria Luigia Ibba, Dongfang Wang, Lucrezia Rinaldi, Simone Ummarino, Giulia Gaggi, Marta Borchiellini, Piotr M. Swiderski, Menotti Ruvo, Silvia Catuogno, Alexander K. Ebralidze, , Annalisa Di Ruscio

This paper develops an RNA-based aptamer, aptaDiR, that neutralizes DNMT1 function to block DNA methylation and impair cancer cell viability.

Nature Communications · Jan 6, 2023Peer-reviewedMolecular Biology

NAD modulates DNA methylation and cell differentiation

Simone Ummarino, Clinton Hausman, Giulia Gaggi, Lucrezia Rinaldi, Mahmoud Adel Bassal, Yanzhou Zhang, Andy Joe Seelam, Ikei S. Kobayashi, Marta Borchiellini, Alexander K. Ebralidze, Barbara Ghinassi, Bon Q. Trinh, Susumu S. Kobayashi, Annalisa Di Ruscio

This study shows that NAD affects DNA methylation and cell differentiation by promoting ADP-ribosylation of DNMT1, leading to CEBPA activation and myeloid differentiation.

Cells · Nov 2, 2021Peer-reviewedMolecular Biology

Formation and recycling of an active epigenetic mark mediated by cell cycle-specific RNAs

Alexander K. Ebralidze, Simone Ummarino, Mahmoud Adel Bassal, Haoran Zhang, Bogdan Budnik, Emanuele Monteleone, Dennis Kappei, Yanjing V. Liu, Danielle E. Tenen, Rory Coffey, Mee Rie Sheen, Yanzhou Zhang, Anaïs Wanet, Bon Q. Trinh, Valeria Poli, , Daniel Geoffrey Tenen

The paper shows that cell cycle-specific long noncoding RNAs regulate the establishment and recycling of the acetylated histone H2A.Z epigenetic mark.

bioRxiv · Oct 27, 2021 · PreprintGenetics

S-Phase induced RNAs control ORC1 engagement to H2A.Z and firing of early DNA replication origins.

Simone Ummarino, Larysa Poluben, Alexander K. Ebralidze, Ida Autiero, Yhanzou Zhang, Theodore Paniza, Madhura Deshpande, Johnathan Lee, Mahmoud Adel Bassal, Bon Q. Trinh, Steven P. Balk, Robert C. Flaumenhaft, Jeannine Gerhardt, Sergei M. Mirkin, Daniel Geoffrey Tenen, Annalisa Di Ruscio

The paper identifies S-phase RNAs that anchor ORC1 to H2A.Z, enabling DNA replication initiation and revealing an RNA-mediated mechanism for replication origin formation.

bioRxiv · Oct 19, 2021 · PreprintMolecular Biology

Targeted intragenic demethylation initiates chromatin rewiring for gene activation

Yanjing V. Liu, Mahmoud Adel Bassal, Migara Kavishka Jayasinghe, Quy Xiao Xuan Lin, Chan-Shuo Wu, Jing Ping Tang, Junsu Kwon, Qiling Zhou, Hong Kee Tan, Alexander K. Ebralidze, Minh T. N. Le, Li Chai, Touati Benoukraf, Annalisa Di Ruscio, Daniel Geoffrey Tenen

CRISPR-DiR induces locus-specific demethylation and chromatin remodeling to reactivate the p16 gene.

bioRxiv · Jul 16, 2020 · PreprintGenetics

Deletion of calcineurin from GFAP-expressing astrocytes impairs excitability of cerebellar and hippocampal neurons through astroglial Na+/K+ ATPase.

Laura Tapella, Teresa Soda, Lisa Mapelli, Valeria Bortolotto, Heather Bondi, Federico Alessandro Ruffinatti, Giulia Dematteis, Alessio Stevano, Marianna Dionisi, Simone Ummarino, Annalisa Di Ruscio, Carla Distasi, Mariagrazia Grilli, Armando A. Genazzani, Egidio Ugo D'Angelo, , Dmitry Lim

This study shows that deleting calcineurin in astrocytes impairs neuronal excitability in the cerebellum and hippocampus by reducing Na+/K+-ATPase activity.

Glia · Oct 18, 2019Peer-reviewedNeuroscience