Liam J. Holt

mTOR signalling, not clock gene rhythms, organises daily physiology

This study shows that mTOR signalling, not clock gene rhythms, drives daily physiological variations in mammals and plants.

Generative design of functional disordered regions in a developmental transcription factor

The paper designs functional disordered regions in a transcription factor using biophysical principles to predict chromatin binding function.

ZATELLITE: a toolkit to visualize and manipulate human centromeres in live cells using synthetic zinc fingers

ZATELLITE enables real-time visualization and manipulation of human centromeres in live cells using synthetic zinc fingers without causing DNA damage.

Polarization Increases Nuclear Stiffness in Macrophages Despite Reduction in Lamin A/C Levels

This study shows that pro-inflammatory polarization of macrophages increases nuclear stiffness despite reduced lamin A/C levels, suggesting chromatin changes drive nuclear mechanics.

Nascent protein retention at polysomes reduces kinetic barriers to self-assembly

The paper shows that increasing translation initiation rates accelerates nucleation of aggregating proteins at polysomes, suggesting cotranslational nucleation influences proteome evolution.

Herpes simplex virus-1 fluidizes the nucleus enabling condensate formation

This study shows HSV-1 protein ICP4 increases nuclear fluidity to enable condensate formation, supporting viral replication.

Quiescence improves Candida albicans survival of fungicidal drug exposure

This study shows that quiescence in Candida albicans enhances survival during antifungal drug exposure by altering cellular properties and gene expression.

Transcriptional consequences of herpes simplex virus 1 ICP4 inducible expression in uninfected cells

This study examines how HSV-1 ICP4 expression in uninfected cells alters host gene expression through RNA-Seq, identifying differentially expressed genes and validating findings against infected cell data.

The Deformability of the Mammalian Cell Nucleus is Determined by the Identity of the Lamin Rod Domain

This study identifies the A-type lamin rod domain as the key determinant of nuclear deformability, revealing its unique role in resisting large mechanical forces on the nucleus.

LINE-1 ribonucleoprotein complexes bind DNA to enable nuclear entry during mitosis

Macromolecular interactions and geometrical confinement determine the 3D diffusion of ribosome-sized particles in live Escherichia coli cells

This study tracks ribosome-sized particles in E. coli to show that their 3D diffusion is influenced by macromolecular interactions and geometrical confinement, revealing sub-diffusive motion due to nucleoid and cell boundary constraints.

Giant KASH proteins and ribosomes synergistically establish cytoplasmic biophysical properties in vivo

The paper identifies two conserved mechanisms controlling cytoplasmic macromolecular diffusion in C. elegans tissues, involving ribosome concentration and the giant KASH protein ANC-1.

The phase separation landscape of genome-wide genetic perturbations

The paper identifies 68 yeast genes that globally influence protein phase separation through genetic knockouts and synthetic biology.

Development and Characterization of 50 nanometer diameter Genetically Encoded Multimeric Nanoparticles

This paper develops and characterizes 50 nm diameter genetically encoded multimeric nanoparticles (GEMs) for improved nanorheology studies.

KaryoTap Enables Cost-Effective High-Throughput Single-Cell Aneuploidy Profiling and Reveals How Positive and Negative Selection Shapes Cancer Genomes

KaryoTap enables high-throughput single-cell aneuploidy profiling, revealing that positive and negative selection shape cancer genomes by influencing chromosomal gains and losses.

SWI/SNF senses carbon starvation with a pH-sensitive low complexity sequence

The paper shows that the SWI/SNF complex uses a pH-sensitive low complexity sequence to sense carbon starvation in yeast.

Synthetic Condensed-Phase Signaling Expands Kinase Specificity and Responds to Macromolecular Crowding

The paper shows synthetic condensates enhance kinase activity and specificity, revealing principles of phosphorylation dynamics in response to macromolecular crowding.

Synthetic genomic reconstitution reveals principles of mammalian Hox cluster regulation

The paper uses synthetic genomic reconstitution to study Hox cluster regulation, finding that distal enhancers are required for full transcriptional output while chromatin remodeling can occur without them.

Comprehensive scanning mutagenesis of human retrotransposon LINE-1 identifies motifs essential for function

The paper identifies functionally important regions in ORF1p and ORF2p of human LINE-1 through trialanine mutagenesis, showing most mutants lose retrotransposition activity.

Ancestral resurrection reveals mechanisms of kinase regulatory evolution

The paper uses ancestral resurrection to show that changes in the β3-C loop and gatekeeper residue in ERK kinases drove the evolution of lower intrinsic autophosphorylation.

Synthetic-evolution reveals that phosphoregulation of the mitotic kinesin-5 Cin8 is constrained

This study shows that phosphoregulation of the mitotic kinesin-5 Cin8 requires site-specific and precise phosphorylation sites to achieve optimal regulation.

The largest SWI/SNF polyglutamine domain is a pH sensor

The paper shows that a polyglutamine domain in the yeast SWI/SNF complex functions as a pH sensor for transcriptional regulation during carbon starvation.