Paolo Mita

Pan-cancer analysis reveals context-dependent roles of LINE-1 ORF1p in immune regulation and copy number alterations

The paper analyzes ORF1p expression across cancers, finding it correlates with immune suppression in some tumors and activation in others, linked to copy number alterations.

Functional high capacity exosome-encapsulating bioinspired hydrogel promotes microvascularbed expansion in diabetic mice

The paper introduces Exo-Q, a hydrogel that promotes microvascular expansion in diabetic wounds, reducing closure time compared to non-diabetic wounds.

Human to yeast pathway transplantation: cross-species dissection of the adenine de novo pathway regulatory node

The paper transplants the human adenine de novo pathway into yeast to study regulatory mechanisms, finding that PPAT/ADE4 is a central regulatory node influenced by metabolites and mutations.

Comprehensive scanning mutagenesis of human retrotransposon LINE-1 identifies motifs essential for function

The paper identifies functionally important regions in ORF1p and ORF2p of human LINE-1 through trialanine mutagenesis, showing most mutants lose retrotransposition activity.

Dissection of purified LINE-1 reveals distinct nuclear and cytoplasmic intermediates

The paper identifies distinct nuclear and cytoplasmic intermediates of LINE-1 retrotransposition using mass spectrometry and affinity purification.

A toolbox of immunoprecipitation-grade monoclonal antibodies against human transcription factors.

The paper develops a collection of 1406 validated monoclonal antibodies against human transcription factors and identifies factors contributing to their successful generation.