Jeffrey Alan Chao

Quantitative comparison of methodologies for translation site imaging in living cells

This study compares translation site imaging methods in living cells, finding that the ALFA-tag minimizes mRNA expression perturbations and enhances fluorescent signal.

Single-mRNA imaging and modeling reveal coupled translation initiation and elongation rates

The paper uses single-mRNA imaging and modeling to show that translation initiation and elongation rates are coupled, maintaining low ribosome density and translational homeostasis.

The One Click Wonder: a retrained automated segmentation pipeline that enables quantitative and modular analysis of C. elegans embryos

The paper introduces OCW, an automated segmentation pipeline for C. elegans embryos, and BAAM for single-cell quantitation, revealing distinct cell populations with varying transcriptional burst frequencies.

Stress granules formed during different RNA virus infections show remarkable plasticity and substantial virus-specific differences in their formation and composition

This study compares stress granule formation and composition during MHV and SFV infections, revealing virus-specific differences in their proteomes, kinetics, and RNA content.

A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C regulates oskar mRNP composition and transport

The paper shows Staufen directly interacts with Tm1 to regulate oskar mRNA transport by modulating kinesin-1 activity in Drosophila oocytes.

Distinct roles of LARP1 and 4EBP1/2 in regulating translation and stability of 5'TOP mRNAs

This study investigates the roles of LARP1 and 4EBP1/2 in regulating translation and stability of 5'TOP mRNAs using single-molecule imaging, showing 4EBP1/2's dominant repression and LARP1's selective protection.

deepBlink: Threshold-independent detection and localization of diffraction-limited spots

deepBlink is a neural network method for automatically detecting and localizing diffraction-limited spots, outperforming existing methods on six datasets.

Single-molecule imaging reveals the coupling of translation and mRNA decay

This study uses single-molecule imaging to show that translating mRNAs decay faster than non-translating ones, with miRNAs mediating efficient degradation of both.

Single-molecule imaging of mRNA localization and regulation during the integrated stress response

The paper uses single-molecule imaging to study mRNA localization in stress granules and processing bodies during the integrated stress response and finds similar translation and degradation rates for mRNA in these compartments compared to cytosol.