Luca Giorgetti

Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts

This study shows that enhancer position within a cis-regulatory landscape modulates transcriptional burst frequency and uniformity in single cells.

Genome-wide absolute quantification of chromatin looping

This study calibrates Micro-C with live imaging to determine absolute chromatin looping probabilities in mouse cells, finding most loops occur at low frequencies.

Spatially correlated fluctuations govern relative chromatin motion

The paper shows spatially correlated fluctuations slow relative chromatin motion in living cells, affecting gene regulation.

Loop extrusion creates rare, long-lived encounters underlying enhancer-promoter communication

The paper identifies rare, long-lived chromatin encounters driven by cohesin-mediated loop extrusion as key to enhancer-promoter communication and shows they explain transcriptional dynamics.

Single-molecule chromosome tracing reveals a diversity of megabase heterochromatin domains

The paper identifies three types of megabase-scale heterochromatin domains in C. elegans using single-molecule tracing and shows their developmental dynamics and structural differences.

Dosage sensitivity of the loop extrusion rate confers tunability to genome folding while creating vulnerability to genetic disruption

The paper shows that the extrusion rate of cohesin is tunable by cofactor dosage and buffers chromosome structure against genetic disruption.

Inversion of a topological domain leads to restricted changes in its gene expression and affects inter-domain communication

The paper inverts a topological domain and shows it causes limited gene expression changes but affects inter-domain communication, particularly in Xist expression.

Nonlinear control of transcription through enhancer-promoter interactions

The paper quantifies how enhancer-promoter interactions influence transcription through nonlinear relationships and reveals mechanisms of transcriptional control via chromosome structure.

Live-cell imaging and physical modeling reveal control of chromosome folding dynamics by cohesin and CTCF

The paper uses live-cell imaging to show that chromosomal contacts within TADs are dynamic and stabilized by CTCF and cohesin.

deepBlink: Threshold-independent detection and localization of diffraction-limited spots

deepBlink is a neural network method for automatically detecting and localizing diffraction-limited spots, outperforming existing methods on six datasets.

A symmetric toggle switch explains the onset of random X inactivation in different mammals

The paper identifies a symmetric toggle switch as the mechanism for random Xist up-regulation during X-chromosome inactivation in mammals.

Modeling of DNA methylation in cis reveals principles of chromatin folding in vivo in the absence of crosslinking and ligation

The paper introduces damC, a method to measure chromatin structure in vivo without crosslinking or ligation, confirming TADs and CTCF loops and showing chromatin rigidity below 5 kilobases.