Daniel Schraivogel

Time-resolved single-cell screening reveals the dynamic regulatory architecture of human cardiogenesis

This study uses time-resolved single-cell CRISPR screening and a multiome atlas to uncover dynamic gene regulatory networks in human cardiogenesis, identifying stage-specific regulators and CHD-linked gene functions.

Image-based transposon screening reveals a flavin reductase that restrains intracellular Salmonella replication in macrophages

The paper uses image-based transposon screening to identify a flavin reductase that promotes Salmonella replication in macrophages by reducing oxidative and nitrosative stress.

Nuclear size is genetically controlled and influences cell fate

The paper identifies regulators of nuclear-to-cell size ratio and shows nuclear size influences gene expression and cell fate.

Cell organization and disturbance-mapping using image activated cell-profiling (CODIAC)

CODIAC profiles spatial protein localization and abundance changes using image-enabled sorting and machine learning, detecting perturbation effects on the spatial proteome.

Multimodal phenotyping defines variant-to-function maps for RBM20 in dilated cardiomyopathy

The paper develops a deep mutational scanning framework to map variants to molecular functions in RBM20, identifying functional hotspots and therapeutic strategies for dilated cardiomyopathy.

Genome-scale mapping of variant, enhancer and gene function in primary human CD4+ T cells

The paper maps variant, enhancer, and gene function in primary human CD4+ T cells by combining Perturb-seq of regulatory elements and genes, identifying CRE-gene pairs and linking variants to disease-related programs.

Plasmodium falciparum egress disrupts endothelial junctions and activates JAK-STAT signaling in a microvascular 3D blood-brain barrier model

This study shows that Plasmodium falciparum egress products disrupt BBB endothelial junctions and activate JAK-STAT signaling, leading to increased permeability and suggesting therapeutic targets.

Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.