Krzysztof Kucharz

Spatiotemporally distinctive astrocytic and neuronal responses to repetitive intracortical microstimulation

This study shows that astrocytes and neurons respond differently to intracortical microstimulation, with astrocytes showing spatially heterogeneous and temporally variable responses.

Impaired Calcium Signaling in Precapillary Sphincters and Pericytes Perturbs Neurovascular Regulation after an Ischemic Stroke

This study investigates how impaired calcium signaling in precapillary sphincters and pericytes contributes to neurovascular uncoupling after ischemic stroke in mice.

Two-photon microscopy of brain endothelial glycocalyx uncovers spatial heterogeneity, vesicular transport, and lectin-binding kinetics in the living brain

This study uses two-photon microscopy to reveal structural and dynamic features of the brain endothelial glycocalyx, including spatial heterogeneity, vesicular transport, and lectin-binding kinetics in vivo.

Single-Cell and Population-Level Neuromodulation Dynamics in Dual-Electrode Intracortical Stimulation

This study investigates how dual-electrode intracortical stimulation modulates neural activity in mice, finding intensity- and spatial-dependent neuromodulation effects.

Vascular dysfunction in Huntington's disease is located at the blood-CSF barrier and is rescued by sphingosine-1-phosphate receptor agonist

This study identifies vessel-type-specific vascular barrier dysfunction in Huntington's disease and shows partial rescue with an S1PR1 agonist.

Multi-omic analysis reveals the unique glycan landscape of the blood-brain barrier glycocalyx

The paper analyzes the BBB glycocalyx using multi-omic approaches, revealing unique glycan composition and structural features, and their potential role in neuroinflammation.

Spatially mismatched cerebral blood flow and neuronal activity by intracortical microstimulation

This study investigates the spatial and temporal mismatch between neuronal activity and blood flow responses to intracortical microstimulation in mice, finding that low intensity stimulation preserves neurovascular coupling while high intensity causes mismatched responses and potential ischemia.

Two-photon microscopy in vivo reveals brain vessel type-specific loss of glycocalyx caused by apoM/S1P signaling impairment.

The paper uses two-photon microscopy to show that S1P signaling impairment causes glycocalyx loss in arterioles but not other vessel types in Apom-/- mice.

Post-capillary venules is the locus for transcytosis of therapeutic nanoparticles to the brain.

The paper identifies post-capillary venules as the site for transcytosis of therapeutic nanoparticles across the blood-brain barrier.

Apolipoprotein M-bound sphingosine-1-phosphate regulates blood-brain barrier paracellular permeability and transcytosis

This study shows that apoM-bound S1P regulates BBB permeability and transcytosis, with S1PR1 agonists able to restore permeability in apoM-deficient mice.