This paper introduces a symmetrically dispersed dual-wedge prism design for spectroscopic single-molecule localization microscopy, improving lateral and spectral precision.

npj Imaging · Mar 31, 2026Peer-reviewedBioengineering

Freeform robotic optical coherence tomography beyond the optical field-of-view limit

Raymond S. Fang, Pengpeng Zhang, Tingwei Zhang, Zihang Yan, Daniel Kim, Edison Sun, Roman V. Kuranov, Junghun Kweon, Alex S. Huang, Hao F. Zhang

This paper introduces a freeform robotic OCT system to overcome the FOV limitation in imaging non-planar anatomies, demonstrating its application in imaging the mouse eye's aqueous humor outflow pathway.

IEEE Transactions on Medical Imaging · Jun 17, 2025Peer-reviewedBioengineering

Robotic Visible-Light Optical Coherence Tomography Visualizes Segmental Schlemms Canal Anatomy and Segmental Pilocarpine Response

Raymond Fang, Pengpeng Zhang, Daniel Kim, Junghun Kweon, Cheng Sun, Alex S. Huang, Hao F. Zhang

This paper uses robotic visible-light OCT to visualize segmental Schlemms canal anatomy and its response to pilocarpine in mice, showing segmental changes in volume.

Investigative Ophthalmology & Visual Science · Feb 18, 2025Peer-reviewedBioengineering

Implementation and calibration of spectroscopic single-molecule localization microscopy

Benjamin Brenner, Wei-Hong Yeo, Youngseop Lee, Junghun Kweon, Cheng Sun, Hao F. Zhang

This paper provides a protocol for implementing and calibrating spectroscopic single-molecule localization microscopy (sSMLM) and introduces a MATLAB package for 3D image reconstruction.

BMC Methods · Feb 10, 2025Peer-reviewedBioengineering

ETV2 primes hematoendothelial gene enhancers prior to hematoendothelial fate commitment

Jeffrey D. Steimle, Chul Kim, Megan Rowton, Rangarajan D. Nadadur, Zhezhen Wang, Matthew Stocker, Andrew Daniel Hoffmann, Erika Hanson, Junghun Kweon, Tanvi Sinha, Kyunghee Choi, Brian L. Black, John M. Cunningham, Ivan P. G. Moskowitz, Kohta Ikegami

This paper identifies sequential phases of ETV2-dependent gene expression that drive hematoendothelial fate commitment from mesoderm progenitors.

Cell Reports · Jun 1, 2023Peer-reviewedDevelopmental Biology

Differential roles of neural crest- and endothelial-derived FOXC2 in trabecular meshwork and Schlemms Canal in glaucomatous pathology

Pieter R. Norden, Lisa Beckmann, Raymond S. Fang, Naoto Ujiie, Zhen Cai, Xian Zhang, Junghun Kweon, Ting Liu, Kazushi Aoto, Susan E. Quaggin, Hao F. Zhang, Tsutomu Kume

The paper investigates the role of FOXC2 in neural crest- and endothelial-derived cells in the trabecular meshwork and Schlemms Canal, showing its importance in development and maintenance of the aqueous humor outflow pathway in glaucoma.

bioRxiv · Feb 20, 2022 · PreprintDevelopmental Biology

A Hedgehog-FGF signaling axis patterns anterior mesoderm during gastrulation

Alexander Guzzetta, Mervenaz Koska, Megan Rowton, Kaelan R. Sullivan, Jessica Jacobs-Li, Junghun Kweon, Hunter A. Hidalgo, Heather Eckart, Andrew Daniel Hoffmann, Rebecca Back, Stephanie Lozano, Anne Moon, Anindita Basu, Michael C. Bressan, Sebastian Pott, Ivan P. G. Moskowitz

This study identifies a Hedgehog-FGF signaling axis critical for anterior mesoderm patterning during gastrulation using single-cell transcriptomics in Hh-deficient embryos.

Proceedings of the National Academy of Sciences · Jun 19, 2020Peer-reviewedDevelopmental Biology