Benjamin Dubreuil

Mapping interactions between disordered regions reveals promiscuity in biomolecular condensate formation

The paper investigates IDR-IDR interactions in synthetic systems to assess their ability to mediate specific condensate formation, finding high promiscuity among some IDRs and specific recognition in others.

A multiplexed approach for genetic screening of human cells by electron microscopy uncovers a critical effector of mitochondrial cristae shape

The paper develops hMultiCLEM, a high-resolution screening pipeline combining EM and multiplexed human cell pools to identify proteins affecting mitochondrial cristae shape, uncovering MISHA linked to Meniere's disease.

Creation and Validation of a Proteome-Wide Yeast Library for Protein Detection and Analysis

The paper creates a proteome-wide yeast library with HA tags to study protein function, size, abundance, and localization.

A pipeline for screening condition-specific enzymes uncovers a function for the alcohol dehydrogenase Bdh2

The paper develops a pipeline to identify condition-specific enzymes in yeast, revealing a function for the uncharacterized Bdh2 alcohol dehydrogenase.

A proteome-wide yeast degron collection for the dynamic study of protein function.

The paper introduces an AID library for on-demand protein depletion in yeast, enabling systematic study of protein function and uncovering new roles in cell cycle and mitochondrial processes.

The phase separation landscape of genome-wide genetic perturbations

The paper identifies 68 yeast genes that globally influence protein phase separation through genetic knockouts and synthetic biology.

An atlas of protein homo-oligomerization across domains of life

The paper predicts homo-oligomerization across four proteomes using AlphaFold2, finding that 50% of archaea, 45% of bacteria, and 20% of eukaryotes form homomers, with disease mutations enriched at interfaces.