This study investigates the dual effects of nicotinamide on aging-related arrhythmia, showing protective effects at low doses and proarrhythmic effects at high doses in mouse and Drosophila models.

Journal of the American Heart Association · May 25, 2026Peer-reviewedPhysiology

Increased ambient temperature mitigates pathology in the Ndufs4-/- mouse model of Leigh Syndrome

Melissa A.E. van de Wal, Christian J. M. I. Klein, Merel J.W. Adjobo-Hermans, Els M.A. van de Westerlo, Clara van Karnebeek, Mirian C.H. Janssen, Jan C. van der Meijden, Judith Regina Homberg, Jaap Keijer, Evert M. van Schothorst, Werner J.H. Koopman

This study shows that raising ambient temperature in Ndufs4-/- mice improves their health outcomes, including longer lifespan and reduced pathology.

bioRxiv · Oct 24, 2025 · PreprintNeuroscience

A novel and robust method for assessing mitochondrial (dys)function in healthy and diseased frozen cardiac tissue

Liangyu Hu, Alexia van Rinsum, Rocco Caliandro, Qi Xi, Shuxiu Fan, Xinyi Jiang, Jur Massop, Melissa Bekkenkamp‐Grovenstein, Christiane Ott, Tilman Grune, Melissa A.E. van de Wal, Werner J.H. Koopman, Marcel Giesbers, Monika M Gladka, Jaap Keijer, Deli Zhang

This paper introduces TSIT, a method to assess mitochondrial function in frozen cardiac tissue, showing comparable results to fresh samples and detecting dysfunction in various mouse models and human samples.

Communications Biology · Aug 19, 2025Peer-reviewedPhysiology

SIRT5 variants from patients with mitochondrial disease are associated with reduced SIRT5 stability and activity, but not with neuropathology

Taolin Yuan, Surinder Kumar, Mary E. Skinner, Ryan Victor-Joseph, Majd Abuaita, Jaap Keijer, Jessica Zhang, Thaddeus J. Kunkel, Yanghan Liu, Elyse M. Petrunak, Thomas L. Saunders, Andrew P. Lieberman, Jeanne A. Stuckey, Nouri Neamati, Fathiya M. Al-Murshedi, , David B. Lombard

The paper identifies two SIRT5 variants in mitochondrial disease patients that reduce protein stability and activity but not neuropathology, using biochemical and mouse model analyses.

bioRxiv · Dec 6, 2023 · PreprintGenetics

OCRbayes: A Bayesian hierarchical modeling framework for Seahorse extracellular flux oxygen consumption rate data analysis

Xiang Zhang, Taolin Yuan, Jaap Keijer, Vincent C. J. de Boer

OCRbayes is a Bayesian framework for analyzing Seahorse XF OCR data, accounting for complex data structures to improve statistical inference.

PLoS ONE · Jul 15, 2021Peer-reviewedSystems Biology