Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR

Jonathan S. Marvin; Benjamin Scholl; Daniel E. Wilson; Kaspar Podgorski; Abbas Kazemipour; Johannes Alexander Müller; Susanne Schoch; Francisco José Urra Quiroz; Nelson Rebola; Huan Bao; Justin P. Little; Ariana N. Tkachuk; Edward Cai; Adam W. Hantman; Samuel S.‐H. Wang; Victor J. DePiero; Bart G. Borghuis; Edwin R. Chapman; Dirk Dietrich; David A. DiGregorio; David Fitzpatrick; Loren L. Looger

doi:10.1038/s41592-018-0171-3

NeuroscienceNature MethodsOct 19, 2018

Peer-reviewed journal article

Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR

Jonathan S. Marvin iD, Benjamin Scholl iD, Daniel E. Wilson iD, Kaspar Podgorski iD, Abbas Kazemipour iD, Johannes Alexander Müller iD, Susanne Schoch iD, Francisco José Urra Quiroz, Nelson Rebola iD, Huan Bao iD, Justin P. Little, Ariana N. Tkachuk iD, Edward Cai, Adam W. Hantman iD, Samuel S.‐H. Wang iD, Victor J. DePiero, Bart G. Borghuis iD, Edwin R. Chapman iD, Dirk Dietrich iD, David A. DiGregorio iD, David Fitzpatrick iD, Loren L. Looger iD

Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter; can detect sub-micromolar to millimolar concentrations of glutamate; and have blue, green or yellow emission profiles. These variants allow in vivo imaging where original-iGluSnFR was too dim, reveal glutamate transients at individual spine heads, and permit kilohertz imaging with inexpensive, powerful fiber lasers.

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