protein folding & dynamics

This paper introduces xGL-APMS to profile endogenous galectin-3 interactions at the cell surface, identifying 125 interactors on Jurkat cells and 69 on mouse thymocytes.

The paper identifies structural adaptations in Microviricetes capsids that enable salinity tolerance by analyzing amino acid residues linked to ocean and lake environments.

The paper compares thick-filament protein ratios in iPSC-derived cardiomyocytes to adult and fetal heart proteomes, finding adult-like stoichiometry despite developmental immaturity.

This study designs de novo amide ligases with a cysteine catalytic triad to selectively form amide bonds across diverse substrates, demonstrating high selectivity and applicability in synthesizing bioactive molecules.

This study shows that NNRTI-induced premature protease activation reduces HIV-1 virion glycoprotein incorporation and infectivity by disrupting Gag lattice and membrane properties.

The paper characterizes LOca-1, a calcium-activated arginyl-specific orthocaspase from Loki's Castle, showing its thermoactivity and structural conservation with plant metacaspases.

The paper introduces a method to identify key players in rare conformational transitions of proteins by analyzing transition corridors and testing their impact through computational and mutational approaches.

This study investigates how ribosome-mediated protein folding imposes sequence constraints by analyzing mutations in E. coli DHFR using deep mutational scanning and fluorogenic sensors.

The paper uses a bioinformatic pipeline to analyze hypothetical proteins in Rickettsia akari, identifying candidates with potential roles in virulence and host interaction.

This study investigates age-related changes in protein insolubility in skeletal muscle and shows that these changes are sex- and compartment-specific and can be modified by exercise and rapamycin.

The paper introduces mE-DIA, a multi-enzyme DIA-MS workflow that improves proteoform detection in proteomics by integrating analysis and interpretation into a single toolset.

This study demonstrates the transferability of homoserine lactone sensors between Bacillales bacteria, enabling programmable interphyla communication between Gram-negative and Gram-positive species.

The paper identifies a conserved cysteine pair motif in CA1Pase linked to redox regulation and inhibitor degradation in Fabaceae species.

This paper introduces a workflow that decouples quantification and identification in label-free top-down proteomics, improving proteome coverage and quantification precision.

The paper introduces a PyTorch-based tool for fast proteome-scale local frustration analysis, achieving 70-13,000x speedup over the original FrustratometeR.

This study identifies extensive horizontal gene transfer in the fungus gnat Bradysia coprophila, showing these genes contribute to stress tolerance and ecological adaptation.

This study shows mutant IDH1 increases sEV secretion and alters their cargo composition in glioma and chondrosarcoma cells, with D2HG packaged into sEVs and cell-type-specific cargo remodeling.

This study identifies the endothelial protein C receptor (EPCR) as the entry receptor for Langya virus and reveals the molecular basis of its interaction with the viral attachment glycoprotein.

This study shows PARP1-mediated PARylation of SPRTN limits its DNA-protein crosslink repair activity by reducing DNA binding and proteolytic function.

This study shows that amyloid structure and condensate stability independently influence the hnRNPA2 condensate-to-fibril transition, revealing a structural framework for this process.

This study shows that fixing whole blood at collection preserves phosphoprotein profiles, particularly in neutrophils and monocytes, better than cryopreserved PBMCs.

This study evaluates the effects of IB-DNQ and Rucaparib on TNBC cells, revealing synergistic effects and novel protein biophysical changes via thermal proteome profiling.

This study shows lipidic α-synuclein fibrils catalyze the formation of mature fibrils through a heterogeneous nucleation mechanism, lowering the energy barrier and following Ostwald's Rule of Stages.

This study maps the Ras/Nore1A binding landscape under different states and mutations, revealing how oncogenic changes alter interaction dynamics and provide insights for inhibitor design.

The paper improves kinase activity inference by refining kinase-substrate relationships with protein-association evidence, enhancing accuracy and coverage in phosphoproteomic data.

This study identifies Jade1S as a SUMO ligase and chaperone that regulates tau stability, prevents oligomerization, and promotes proteasomal degradation of aggregated tau.

The paper develops a scalable assay and a protein language model to predict monomeric yield of proteins in E. coli, showing it can be learned and designed.

This study shows chronic sleep disruption causes distinct transcriptional and proteomic changes in brain compartments, particularly affecting synaptic function and potentially contributing to Alzheimer's risk.

The paper investigates how electrostatic interactions control conformational changes and proton translocation in Complex I using a combination of experimental and computational methods.

This study shows that the intrinsically disordered N-terminal region of eIF5B drives RNA-driven phase separation in vitro and validates this in cells.