Bacterial Genetics and Biotechnology

This study identifies key interactions and functions of the RNase E CTD in Pseudomonas aeruginosa, revealing its role in transcript regulation, virulence, and environmental adaptation.

The paper shows that MdfA and ClpCP protease regulate metabolic differentiation in Bacillus subtilis sporulation by depleting metabolic enzymes in the forespore.

This study identifies CciT and CciO as key proteins involved in iron transport that confer copper resistance in Caulobacter crescentus by modulating cellular iron and copper levels.

This study identifies a gene pair essential for Caulobacter iron homeostasis under EDTA stress and shows its conservation in Proteobacteria.

This paper identifies a new regulator, RocA3, and expands the Roc two-component regulatory system in Pseudomonas species, revealing its evolutionary plasticity and role in virulence.

This study identifies EcpK as a bacterial tyrosine pseudokinase essential for exopolysaccharide biosynthesis in Myxococcus xanthus, showing it functions as a scaffold rather than through phosphorylation.

This study shows that bacterial flagellum assembly is optimized for energy efficiency and motility by balancing secretion rate and filament length.

This study identifies YibN as a functional interactor of YidC, showing it enhances membrane protein insertion and lipid production in bacteria.

This study reports the crystal structure of ZraS-CD from E. coli, revealing conformational changes and interactions critical for autophosphorylation in sensor histidine kinases.

The paper develops a method to detect invertons in gut microbiome metagenomic data and finds associations between inverton dynamics and intestinal colonization and surface adhesion.

The paper identifies unconventional mRNA processing and degradation pathways for the polycistronic yrzI mRNA in B. subtilis, involving translation-dependent cleavage and 3'-exoribonuclease action.

The paper uncouples Tol-Pal complex function in maintaining outer membrane lipid homeostasis from its role in cell division in E. coli.

This study identifies LolB homologs in Bacteroidetes and shows they are involved in gliding motility and Type IX secretion, but lipoprotein transport can occur independently of LolA and LolB.

This study determines the structural basis of MntR-mediated gene activation through cooperative DNA binding using cryo-EM and complementary assays.

The paper shows that ProQ and FinO discriminate RNA based on sequence elements around terminator hairpins, with purine-purine mismatches reducing ProQNTD binding.

This study identifies TamL as essential for outer membrane integrity in Bacteroidetes, showing its role in maintaining membrane stability and interacting with TamB.

This paper evaluates plasmid vectors for gene expression in Pseudovibrio bacteria, showing they can be used for fluorescent protein expression and studying bacterial behaviors.

This study investigates the function of FtsZ in a cell wall-less bacterium, showing that SeFtsZ forms ring-like structures in cell wall-less cells, suggesting a role in cell division independent of cell wall synthesis.

This study characterizes the structure and nucleotide-dependent binding mechanism of E. coli NrdR, revealing its flexibility in adapting to different promoter configurations.

The paper shows that TraE and TraD, inner membrane proteins of the T4SS, bind DNA and identifies potential targets for conjugation inhibitors.

The paper identifies the sRNA InvR as a negative feedback regulator of hilA mRNA translation in Salmonella, fine-tuning SPI1 expression to prevent overactivation.

The paper identifies two pathways for RNA 3' end processing in the gal operon: Rho-dependent termination and RNase E-mediated cleavage, which generate pre-galE mRNA 3' ends that are stabilized by a hairpin structure.

This paper identifies AhCobQ as a novel NAD+- and Zn2+-independent lysine deacetylase in Aeromonas hydrophila with unique domain and substrate specificity.

This study evaluates sigma factor deletions in E. coli to improve recombinant protein production, finding that rpoD and rpoS mutants show enhanced expression in specific media and conditions.

This study shows that (p)ppGpp and DksA reduce the effectiveness of beta-lactam antibiotics by altering bacterial membrane permeability and peptidoglycan biosynthesis in Vibrio cholerae.

This paper shows that bacterial aging, through asymmetric damage inheritance, creates phenotypic heterogeneity that biases persistence rates during antibiotic treatment.

This paper shows that ChiS and TfoS, two transmembrane transcriptional regulators in V. cholerae, work together to activate natural transformation in response to chitin by recruiting the PtfoR promoter to the membrane and facilitating TfoS interaction.

The paper shows that bacterial investment in flagellar motility is constrained by hydrodynamics and fitness costs, with E. coli strains maximizing motility under nutrient-rich conditions.

The paper investigates NrdR repressor DNA binding preferences across species, finding optimal spacer lengths between NrdR boxes for efficient binding.

This study shows that Escherichia coli {rho} forms higher-order oligomers under stress, linking transcription termination to cellular physiology.