Thomas V. O’Halloran

This paper develops a workflow for high-resolution metal imaging in C. elegans using LA-ICP-TOF-MS, achieving 2-m spatial resolution with optimized embedding and ablation techniques.

The paper demonstrates that using an empirically measured blank spectrum improves the accuracy of quantitative X-ray fluorescence mapping by reducing dependencies on dwell time and spectral aggregation.

The paper functionally screens ZIP8 variants to identify pathogenic mutations and trafficking defects using a combined experimental and computational approach.

This study shows hydrogen sulfide increases copper uptake and localization in cells, with copper primarily in the Cu2+ state, using the ZNT1 transporter for import and ATP7A for export.

This study investigates how material composition and implantation site affect in vivo degradation rates of tissue engineering devices using CT-visible radiopaque composites.

The paper calculates photon requirements and radiation dose for X-ray fluorescence microscopy using single excitation energy to detect low-concentration elements in biological tissues.

The paper introduces MAGIC MRI, a gene-based reporter system for non-invasive neural tracing in vivo, validated in rodents and rhesus macaques.

The paper describes a bending magnet beamline with capillary optics for X-ray fluorescence microscopy, comparing spatial resolution and fluence between two microscopy stations.

This study investigates the distinct roles of ATP7A and ATP7B in noradrenergic signaling, showing they have non-redundant functions in neuronal copper homeostasis and metabolic regulation.

The paper investigates the functional distribution of FDX1 and FDX2 in Chlamydomonas, showing their distinct roles in carbon, nitrogen, and sulfur assimilation through mutant analysis.

The paper profiles inorganic elements in mouse embryos and shows zinc supplementation during blastocyst formation increases Nanog expression in the inner cell mass.

The paper identifies ZNG3 as a Zn chaperone interacting with Cia5 to regulate Zn and CO2 metabolism in Chlamydomonas, with distinct phenotypes in ZNG3 and Cia5 mutants.

This study identifies non-redundant roles of ClsA and ClsB in shaping lipid composition and stress resilience in Bacteroides fragilis.

This study identifies dihydropyridines as inhibitors of Brucella ovis growth and shows that pseudogenes like bepE can confer stress resistance through functional restoration.

This paper identifies and characterizes ZpdA, a zinc-regulated phosphodiesterase in V. cholerae that modulates cyclic di-GMP levels through transcriptional and post-translational zinc dependence.

This study shows that AIRE deficiency causes epididymitis and male infertility through immune-mediated fibrosis, iron deposition, and metal imbalance leading to sperm DNA damage.

This study identifies a gene pair essential for Caulobacter iron homeostasis under EDTA stress and shows its conservation in Proteobacteria.

This study modifies key residues in ZIP8 to alter its metal transport activity and substrate spectrum, identifying variants with enhanced activity and expanded substrate preferences.

AutoSpect automates LA-ICP-TOF-MS data processing, addressing drift correction and peak deconvolution to enable accurate elemental mapping and isotopic analysis.

This paper determines the turnover number of human ZIP4 using stable zinc isotopes and ICP-MS, finding it ranges from 0.08 to 0.2 s-1.