Bacterial Genetics and Biotechnology

The paper describes the integration and excision of a 37-kb element in Anabaena variabilis, mediated by a putative integrase.

This study identifies fluoride's mechanism of killing Streptococcus mutans by inhibiting Clp protease, leading to uncontrolled competence cascade and cell lysis.

This study shows that cyclic di-AMP inhibits thymineless death in Listeria monocytogenes during infection, revealing a new regulatory mechanism for bacterial survival under thymidine starvation.

The paper identifies a non-functional variant of a bacterial communication system that rewires gene regulation to enhance stress adaptation and colonization in a globally successful Streptococcus pneumoniae lineage.

This study shows Dps compacts the E. coli nucleoid and increases short-range DNA interactions without significantly affecting chromosome accessibility, dynamics, or organisation.

This study identifies TagF as a checkpoint protein that coordinates spike-loading in T6SS assembly by preventing premature sheath formation and interacting with Hcp and TagZ.

This study investigates the metabolic changes in a spoT-disrupted Acinetobacter baumannii mutant under polymyxin treatment, revealing altered metabolite profiles linked to increased antibiotic susceptibility.

This study shows FliI ATPase is essential for switching substrate specificity and late-stage flagellar assembly in Salmonella, despite being dispensable for early export.

The paper shows that ClpX promotes swarming motility in Bacillus subtilis by degrading Spx, which represses flagellar gene transcription.

The paper uses structural modeling and experimental analysis to reveal how CBP and ChiS regulate chitin utilization in Vibrio cholerae through an allosteric switch mechanism.

The paper identifies the ParB-CTPase fold as a versatile NTP-binding module found across diverse organisms, showing expanded nucleotide specificity and functional diversity through domain fusions.

The paper identifies intrinsic promoter strength as a key factor determining the temporal hierarchy of SOS response gene activation in Caulobacter crescentus, beyond LexA repressor properties.

The paper demonstrates that bacterial flagellar motor stator units are rotary motors, with MotA pentamers rotating faster than the rotor, revealing a gear-like mechanism.

This study uses single-cell RNA-seq to reveal concentration-dependent survival trajectories of Acinetobacter baumannii under antibiotic treatment, uncovering hidden heterogeneity and resistance mechanisms.

This study identifies how ZapA interacts with FtsZ to facilitate Z ring formation in E. coli by using a dual binding mechanism.

The paper identifies a novel archaeal transcription factor family, AmzR, that combines prokaryotic and eukaryotic regulatory features, functioning as a negative regulator of methanogenic growth.

This study shows that Aer in E. coli functions as a bidirectional redox sensor mediating negative chemotaxis to antibiotic-induced ROS and enables bacterial movement away from ROS-generating antibiotics.

This study identifies the biosynthetic genes for glucosaminyl phosphatidylglycerol (GlcN-PG) in Pseudomonas aeruginosa and demonstrates their role in its production and potential transport.

The paper identifies accessory PDFs in bacterial genomes that confer resistance to PDF inhibitors and are mobile, posing a challenge for antibiotic development.

This study identifies pathways in P. aeruginosa that confer resistance to T6SS effectors from Acinetobacter baylyi and Vibrio cholerae, revealing links between T6SS resistance and antibiotic susceptibility.

This study identifies FleQ as a regulator of nrdR in Pseudomonas aeruginosa, showing it activates nrdR under planktonic and infection conditions but represses it during biofilm formation.

The paper proposes the Texas 2-Step model to explain how YcgR modulates flagellar motor rotation in E. coli by interacting with MotA and FliG through conformational changes.

The paper reports the cryoEM structure of TraT, an outer membrane protein involved in plasmid exclusion, and suggests it functions by physical interference rather than specific protein interactions.

The paper shows that SpoVD's catalytic activity is dispensable for asymmetric division in C. difficile due to PBP3's compensatory role, suggesting functional redundancy among bPBPs.

This study shows temperature changes affect PipX interactions in cyanobacteria, revealing PipX as a thermometer for cold shock and demonstrating the NanoBiT system's utility in studying environmental signaling.

The paper integrates RNA-seq data to identify sRNA-mRNA interactions in S. aureus biofilms, prioritizing candidates through differential expression and co-expression analysis.

This study identifies the C-terminal linker of FtsZ as a curvature tuner that scales Z-ring diameter with cell width through charge segregation.

The paper develops two compatible plasmids for studying sRNA-mediated post-transcriptional regulation in Acinetobacter baumannii and validates new sRNA-mRNA interactions.

This paper develops a standardized genetic toolkit for Deinococcus radiodurans, enabling tunable gene regulation and genome engineering with various components for plasmid and chromosomal modifications.

The paper uses cryo-EM and simulations to reveal how LptM and LptY facilitate LPS transport by modulating LptD conformational dynamics in the translocon complex.