Biofuel production and bioconversion

Roles and transcriptional regulation of the endogenous cellulases in association with substrate and cellular processes in a thermoacidophilic archaeon

This study investigates the roles and transcriptional regulation of three cellulases in the thermoacidophilic archaeon Saccharolobus islandicus, revealing regulatory mechanisms and their association with cellular processes.

Genomic and Transcriptomic Characterization of Carbohydrate-Active Enzymes in the Anaerobic Fungus Neocallimastix cameroonii var. constans

This paper characterizes the genomic and transcriptomic features of Neocallimastix cameroonii var. constans, an anaerobic fungus, revealing its capacity to degrade lignocellulose and identifying key carbohydrate-active enzymes.

Discovery and characterization of a copper-binding carbohydrate-binding module (CBM) regulating the activity of lytic polysaccharide monooxygenases

The paper identifies a copper-binding CBM2 module that regulates LPMO activity by scavenging free copper and interacting with the LPMO's catalytic site, preventing oxidative damage.

Hydrothermal pretreatment renders peat susceptible to enzymatic saccharification

This study shows that hydrothermal pretreatment makes peat susceptible to enzymatic saccharification by disrupting cell structure and releasing monosaccharides, but acidification from abiotic reactions inactivates enzymes.

pH adjustment increases biofuel production from inhibitory switchgrass hydrolysates

This study shows that adjusting pH and using SAA pretreatment improves biofuel production from drought-switchgrass hydrolysates.

Lignocellulose Degradation in Bacteria and Fungi for Biomass Conversion

This paper reviews lignocellulose degradation mechanisms in bacteria and fungi, identifies potential cellulosome-producing species, and discusses bioengineering strategies for industrial applications.

A Distinct Autofluorescence Distribution Pattern Marks Enzymatic Deconstruction of Plant Cell Wall

This study identifies a distinct autofluorescence pattern marking enzymatic deconstruction of plant cell walls and develops a computational pipeline to assess hydrolysis impact.

Construction of a low-temperature degrading synthetic bacterial community of corn straw

The paper constructs a synthetic bacterial community that efficiently degrades corn straw at low temperatures, achieving higher degradation rates in both solid-state and liquid-state fermentation.

Rational design paving the way for improving glucose tolerance and catalytic properties of a β-glucosidase from Acetivibrio thermocellus

This paper reports the rational design of β-glucosidase mutants with improved glucose tolerance and catalytic efficiency for bioethanol production.

Rewiring Catalytic Craters: A Path for Engineering β-Glucosidases to Improve Glucose Tolerance

This paper engineers β-glucosidases to improve glucose tolerance by analyzing structural changes and mutations that enhance catalytic efficiency and stability.

PRODUCTION OF BIOETHANOL FROM WATER HYACINTH USING MONOCULTURE AND CO-CULTURE OF MICROORGANISMS

This study investigates bioethanol production from water hyacinth using mono- and co-culture fermentation with Aspergillus niger, Saccharomyces cerevisiae, and Bacillus cereus, finding that co-culture with A. niger and S. cerevisiae yields the highest ethanol concentration.

Effect of Over-expression of GRXs on Thermo and Acetic Acid Stress Tolerance of Saccharomyces cerevisiae

This study shows over-expressing GRX genes improves Saccharomyces cerevisiae growth under thermal and acetic acid stress, enhancing ethanol production potential.

Comparison of microbial and sono-assisted alkaline pre-treatment of sugarcane bagasse, followed by one-pot synthesis of microbial extracellular polymeric substance through simultaneous delignification, saccharification, and fermentation

Simultaneous enumeration of yeast and bacterial cells in the context of industrial bioprocesses

The paper compares methods for simultaneously quantifying yeast and bacterial cells in mixed suspensions, finding that flow cytometry, Coulter counter, and Spread-plate methods are statistically similar for yeast, while microscopy and Drop-plate are similar for bacteria.

Whole genome structural predictions reveal hidden diversity in putative oxidative enzymes of the lignocellulose degrading ascomycete Parascedosporium putredinis NO1

The paper reports the genome of Parascedosporium putredinis NO1, revealing expanded oxidative enzyme repertoires and identifying new lignocellulose-degrading enzymes through structure-based methods.

Targeting lignocellulolytic gene clusters in novel Trichoderma atroviride and Trichoderma harzianum strains through Bac-guided analysis

The paper identifies novel lignocellulolytic gene clusters in Trichoderma species by analyzing genomic regions enriched in degradative enzymes using BAC methodology and RNA-seq data.

Oriented Display of Cello-Oligosaccharides for Pull-down Binding Assays to Distinguish Binding Preferences of Glycan Binding Proteins

The paper develops a pull-down binding assay to assess the directional binding preferences of cellulose-binding modules to cello-oligosaccharides, revealing similar affinity for cellohexaose but not cellopentaose.

Computational investigations in inhibition of alcohol/aldehyde dehydrogenase in lignocellulosic hydrolysates

The paper uses computational methods to study the inhibition mechanisms of alcohol/aldehyde dehydrogenases in lignocellulosic hydrolysates and proposes a methodological framework for enzyme inhibition analysis.

A membrane integral methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases

The paper identifies an N-terminal histidine methyltransferase (NHMT) responsible for methylation of LPMOs in filamentous fungi, showing its role in protecting LPMOs from auto-oxidative inactivation.

Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases

This study uses machine learning to identify sequence features that predict functional subtypes and CBM presence in GH7 glycoside hydrolases with high accuracy.

Whole Genome Sequence and CAZyme distribution of the cellulase hyper producing filamentous fungus Penicillium janthinellum NCIM 1366

The paper sequences the genome of Penicillium janthinellum NCIM 1366 and compares its CAZyme content to Trichoderma reesei RUT-C30, finding a higher number of CAZymes in P. janthinellum which may explain its superior cellulolytic efficiency.

The synergistic actions of hydrolytic genes reveal the mechanism of Trichoderma harzianum for cellulose degradation

This study identifies synergistic gene and protein interactions in Trichoderma harzianum for cellulose degradation using transcriptomic and proteomic analyses.

Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30

The paper analyzes gene regulatory networks in Trichoderma reesei under glucose-lactose mixtures to understand cellulase production regulation.

Integrative Genomic Analysis for the Bioprospection of Regulators and Accessory Enzymes Associated with Cellulose Degradation in a Filamentous Fungus (Trichoderma harzianum)

The paper identifies CAZymes, transcription factors, and transporters in Trichoderma harzianum IOC3844 involved in cellulose degradation using genomic, transcriptomic, and exoproteomic data.

Targeted biomass degradation by the higher termite gut system - integrative omics applied to host and its gut microbiome

This study investigates how a higher termite adapts to a Miscanthus diet using integrative omics, revealing shifts in microbial gene expression and potential industrial enzyme sources.

Enabling community-based metrology for wood-degrading fungi

The paper establishes standardized methods and reference materials for measuring wood-degrading fungi growth to enable reproducible and scalable bioproduction.

Genome-centric portrait of the microbes' cellulolytic competency

The paper develops a pipeline to predict cellulolytic competency in microbes by analyzing genome annotations and synergy machineries, identifying five groups based on carbohydrate active modules.

Bioethanol from Sago Waste Fermented by Baker's and Tapai Yeast as a Renewable Energy Source

This study produces bioethanol from sago waste using baker's yeast and tapai yeast through fermentation, finding that baker's yeast fermentation yields the highest ethanol content.

Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables thermophilic isobutyl acetate production directly from cellulose by Clostridium thermocellum

This study engineers a thermostable chloramphenicol acetyltransferase to enable isobutyl acetate production from cellulose by Clostridium thermocellum at elevated temperatures.