Advanced Fluorescence Microscopy Techniques

The paper develops iATPSnFR2, a high dynamic range fluorescent ATP sensor for real-time subcellular ATP monitoring, showing improved performance over previous versions.

The paper introduces PECAbs for high-specificity multiplexed imaging of cell states and combines them with seq-smFISH to classify cells and identify signal activation states in human tissue.

This paper describes a U-ExM protocol for analyzing subcellular structures in mouse and human kidney tissues with high detail using conventional microscopy.

This paper provides a protocol for setting up high-end 3D multi-color single-molecule localization microscopy (SMLM) in a laboratory setting.

This study uses fluorescence lifetime imaging to quantify metabolic heterogeneity in colorectal cancer, finding higher heterogeneity in patient tumors compared to cell lines and xenografts, with potential prognostic value.

This paper introduces ExFLPAM, a method that expands the capabilities of expansion microscopy by enabling on-demand fluorescent labeling and non-fluorescent imaging in live cells and tissues.

This paper introduces ARES, a baseline-free method for quantifying fluorescence signals that improves spatiotemporal resolution of calcium imaging data and enables tracking of intracellular calcium dynamics.

This paper introduces EC-STORM, an electrochemical method to control fluorophore blinking for STORM imaging, improving molecular counting and imaging quality over conventional photochemical STORM.

OneFlowTraX is a user-friendly software for analyzing single-molecule dynamics and nanoscale organization in living cells, offering automated localization, tracking, and clustering analysis.

This paper introduces a 3D orientation mapping microscope that achieves high temporal-spatial resolution for fluorescent dipoles, revealing molecular structure and dynamics in biological systems.

This paper describes surface passivation and functionalization methods for interferometric scattering microscopy to reduce non-specific binding and enable single-molecule experiments.

This study evaluates 16 fluorescent proteins in Salmonella enterica to identify optimal tools for monitoring cellular processes, highlighting the benefits of degradation tags for improving signal stability and reducing cell-to-cell variability.

This paper introduces a multimodal illumination platform for 3D single-molecule super-resolution imaging in mammalian cells, improving signal-to-background ratio and localization precision.

The paper develops T-GECO1, a blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon brightness for improved neuronal activity imaging.

The paper uses molecular dynamics simulations to analyze hydrogen bond dynamics in a maltose biosensor, identifying key factors distinguishing its bright and dark states.

The paper introduces an improved open-source light-sheet microscope, mesoSPIM, with enhanced resolution, throughput, and compatibility with cleared samples.

This study develops a highly sensitive GEVI called rEstus to measure electrical activity in non-excitable cells and demonstrates its use in detecting voltage fluctuations and gap-junction coupling.

The paper develops ultrasensitive cAMP and Ca2+ indicators to measure their dynamics in neurons and identifies their roles in signaling and behavior.

The paper develops a non-invasive NIR imaging technique to visualize live plant cell nuclei using phytochrome-derived autofluorescence, applicable across multiple plant species.

The paper describes an open-source fluorescent imaging system (openIVIS) that can be used for in vivo and in vitro applications, demonstrating its versatility and low cost.

This paper introduces a beam-based focus stabilisation method using astigmatism, compatible with various objective lenses and offering nanometre precision and extended operating range.

The paper introduces MINSTED localization, a method for tracking labeled biomolecules with nanometer and millisecond precision using STED microscopy.

The paper derives a depth-dependent axial scaling theory for light microscopy under refractive index mismatch and provides software to correct axial deformation in imaging data.

This paper develops an optogenetic method to release single molecules in live cells, enabling controlled delivery of functional proteins and reconstitution of cellular functions at the single molecule level.

This paper introduces PPG3D, a PCA-based PSF generator for 3D localization microscopy, improving accuracy and speed for large FOV imaging.

This study compares the photostability of StayGold with three other FPs under different illumination modes in microscopy, finding that StayGold maintains superior photostability in multi-beam LSCM but shows variable performance in single-beam LSCM.

This paper introduces a 4Pi MINFLUX technique that achieves high spatio-temporal localization precision for single fluorophores, revealing nanoscale diffusion heterogeneities in solutions.

This paper develops O2-independent bioreporters using KOFP-7, demonstrating fluorescence in anaerobic conditions for bacteria like V. diazotrophicus.

This paper introduces SUM-PAINT, a high-throughput imaging method for single-protein spatial resolution, and discovers a new synapse type through extensive multiprotein analysis.

This paper presents an oblique plane microscope compatible with a commercial base, showing minimal impact on resolution and data fidelity despite objective lens displacement.