Advanced Fluorescence Microscopy Techniques

Open Blink: Low-cost TIRF microscopy for super-resolutionimaging via μManager

Open Blink provides a low-cost, open-source TIRF microscope for quantitative multi-color super-resolution imaging with high localization precision and axial stability.

NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding

This paper introduces NanoBlocks, a method to convert affinity binders into fluorescent biosensors by adding competitive peptides, demonstrating their use in detecting targets in vitro and in cells.

Quantitative imaging of calcium dynamics with a green fluorescent biosensor and fluorescence lifetime imaging

The paper describes protocols for calcium imaging using a genetically encoded biosensor and fluorescence lifetime imaging to improve measurement robustness.

Cellular-resolution Three-photon Ultra-Deep Imaging (TrUDI) of neuronalpopulation activity at 2 mm depth in the mammalian brain

The paper introduces TrUDI, a three-photon microscopy system that improves imaging depth in the mouse brain to ~2 mm with enhanced fluorescence detection.

Continuous mass photometry by single molecule trapping

This paper introduces a method for continuous mass photometry of single molecules, enabling long-term observation of biomolecular interactions with high mass precision and temporal resolution.

Red fluorescent labeling of myelin by membrane-targeted tdTomato in transgenic mouse lines

The paper develops transgenic mouse lines with red fluorescent labeling of myelin, enabling structural and functional imaging compatible with green spectral sensors.

Topology-dependent FRET efficiency in living cells via N-C swapping of fluorescent protein fusions

This study shows that the topology of fluorescent protein fusions, not just distance, affects FRET efficiency in living cells, with donor-acceptor configurations showing higher efficiency than acceptor-donor.

Using iPALM to determine protein organisation in cardiac muscle Z-discs

The paper uses iPALM to determine the 3D organisation of three proteins in cardiac muscle Z-discs, revealing differences in their arrangement.

Third Harmonic Generation Microscopy Reveals Structure and Mucus Dynamics in Human Airway Epithelium Models

This paper uses third-harmonic generation microscopy to visualize and measure mucociliary transport in human airway epithelium models without staining or processing.

Self-supervised Internal Learning Enhances Isotropic Resolution for Three-dimensional Fluorescence Microscopy

This paper introduces DeepIso, a self-supervised framework that enhances isotropic resolution in 3D fluorescence microscopy by restoring axial frequency content without requiring explicit PSF models or system calibration.

Precise Alternation Between Image-Forming Sample Planes Enables Quantitative Monitoring of Receptor-Arrestin Interaction Dynamics at the Plasma Membrane of Live Cells

The paper introduces an optical imaging method to precisely monitor receptor-arrestin interactions in live cells, showing arrestin redistribution upon receptor activation.

A practical framework for measuring protein oligomerization equilibria by fluorescence correlation spectroscopy

This paper provides a framework for measuring protein oligomerization equilibria using fluorescence correlation spectroscopy, demonstrating its application with PCNA under varying ionic strength conditions.

Quantifying the spatio-temporal image degradation under motion blur in fluorescence microscopy

This study quantifies spatio-temporal image degradation from motion blur in fluorescence microscopy using SSNR and simulations, showing resolution loss up to 40% for 90-degree motion direction changes.

Broadband backscattering confocal microscopy enables label-free 3D live cell nanoscale sensitive imaging

This paper introduces broadband backscattering confocal microscopy (BBCM) for label-free 3D live cell imaging with improved contrast and size sensitivity.

PSF-Driven Spatio-Temporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.

This paper introduces a method to mitigate spatio-temporal blending in FLIM by using MSSR-based masking, improving spatial resolution while preserving lifetime accuracy.

Autofluorescence lifetime imaging resolves cell heterogeneity within peripheral blood mononuclear cells

This paper demonstrates that autofluorescence lifetime imaging can identify immune cell subsets and activation states in PBMCs with high accuracy, enabling label-free metabolic analysis.

Characterizing particle dynamics in live imaging through stochastic physical models and machine learning

This paper develops a machine learning method to classify subdiffusive particle dynamics in live imaging, showing it can distinguish different motion behaviors and reveal how receptor dynamics relate to HIV-1 activity.

Counting fluorescent emitters with a single photon avalanche diode array

This paper introduces a method using SPAD arrays to count fluorophores in widefield microscopy by modeling PCH statistics and performing Bayesian inference.

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy.

This paper demonstrates that self-blinking dye JF635b improves nanobody performance in single-molecule localization microscopy, enabling reliable imaging across multiple modalities with high precision.

Single-dye, transfection-free FLIM multiplexing via bioorthogonal chemistry

The paper demonstrates a method to multiplex FLIM using small molecule probes modified with bioorthogonal chemistry to achieve distinct fluorescent lifetimes without transfection.

Development of a photostable pH biosensor based on mStayGold

The paper develops a photostable pH biosensor called serapH using mStayGold as a scaffold and introduces a screening method for pH-sensitive FPs in bacterial colonies.

Dodecagon light-sheet fluorescence microscopy for large-volume imaging without striping artifacts

This paper introduces dodecagon light-sheet fluorescence microscopy (dodecaLSFM) to eliminate striping artifacts in large-volume imaging, demonstrating its effectiveness in imaging whole mouse brain vasculature and combining with expansion microscopy for 3D cellular resolution.

A non-toxic, user-friendly buffer that enhances green fluorophore performance in conventional and super-resolution imaging

The paper introduces a non-toxic buffer that enhances green fluorophore brightness and photostability in both conventional and super-resolution imaging.

Experimental and simulated FRAP for the quantitative determination of protein diffusion in helical cells

The paper develops a framework for quantitatively determining protein diffusion in helical cells using FRAP simulations and experiments, finding similar diffusion rates in helical and rod-shaped bacteria.

Space-Time Light-Sheet Microscopy

This paper introduces space-time light-sheet microscopy (ST-LSM), a single-objective technique that improves imaging volume and field of view while maintaining resolution.

Calibration-free single-frame super-resolution fluorescence microscopy

This paper introduces a deep-learning method for super-resolution fluorescence microscopy that reconstructs images from a single camera frame without calibration or long acquisition times.

Imaging the voltage of neurons distributed across entire brains of larval zebrafish

The paper presents a remote scanning light-sheet microscope for whole-brain voltage imaging in larval zebrafish, revealing spatial patterns of neuronal activity during visual stimuli and spontaneous bursts.

aDISCO: A clearing method to enable 3D microscopyof large archival paraffin-embedded human tissue blocks

aDISCO enables 3D microscopy of large archival FFPE human tissue blocks, revealing subtle pathologies in focal cortical dysplasia through deep-learning analysis.

Genetic encoding of 3-cyano-tyrosine and its use in controlling the chromophore isomeric state of the fluorescent protein mKate

This paper genetically encodes 3-cyano-L-tyrosine into a fluorescent protein, altering its chromophore isomerization state and spectral properties.

Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy

This paper introduces sDNA-PAINT, a method that improves spectral precision and multiplexing in super-resolution microscopy using DNA Origami Nanorulers and spectroscopic SMLM.