Bacterial biofilms and quorum sensing

A microbiome-derived metabolite from Staphylococcus epidermidis inhibits Staphylococcus aureus biofilm formation and virulence

This study identifies pyroglutamic acid as a metabolite from S. epidermidis that inhibits S. aureus biofilm formation and virulence in vitro and in vivo.

AutoWrinkleID: a machine learning pipeline for biofilm wrinkle identification and quantitative analysis

AutoWrinkleID is a machine learning pipeline for automated biofilm wrinkle detection and quantification, showing that motility-based annotations outperform manual and constitutive fluorescence annotations.

Evolutionary expression patterns in developing biofilms of uropathogenic Escherichia coli reveal embryo-like features

This study shows that uropathogenic E. coli biofilms exhibit evolutionary stratification, with gene expression becoming younger and more divergent as biofilms develop.

From planktonic to sedentary lifestyle: Molecular dissection of the establishment and maintenance of mycobacterial biofilm

This study examines molecular changes in mycobacterial biofilm development using transcriptomic and proteomic analyses, revealing distinct gene expression and protein profiles during biofilm establishment and maturation.

Host nutrients drive paired-substrate growth and distinct biofilm lifestyles in Finegoldia magna

This study shows that Finegoldia magna has a restricted metabolism dependent on specific host nutrients, which also influence its biofilm behavior.

The Influence of Material Surface Properties on Initial Bacterial Attachment and Biofilm Formation on Built Environment Surfaces

Across seven built-environment materials, initial attachment efficiency of four bacteria depended on species (Gram-negative higher than Gram-positive), not material, whereas 24-hour biofilm biomass was highest on stainless steel.

Temperature-dependent reprogramming of virulence traits during Pseudomonas aeruginosa biofilm formation

This study examines how temperature affects Pseudomonas aeruginosa virulence traits and biofilm properties during growth at different temperatures.

Colony morphogenesis regulates sporulation dynamics in bacterial biofilms

This study shows that colony expansion and sporulation dynamics in Bacillus subtilis biofilms are interdependent, with sporulation waves driving dormancy while non-sporulating cells maintain expansion.

Biophysical properties of material-colonizing fungal biofilms: multiscale structural and mechanical characterization

This paper develops a multiscale methodological workflow to characterize the structural and mechanical properties of fungal biofilms formed by Knufia petricola, revealing how nitrogen source affects biofilm morphology and stiffness.

Biofilms promote tolerance and stability in gut bacterial communities during bile acid stress

This study investigates how biofilms in multispecies gut microbiota communities enhance tolerance and stability during bile acid stress.

Biofilm formation and dynamics in the marine cyanobacterium Prochlorococcus

This study shows Prochlorococcus forms biofilms, transitions between planktonic and biofilm states, and exudes more organic carbon, suggesting a role in deep-sea carbon export.

Host immune stress reveals a role for potassium homeostasis in Pseudomonas aeruginosa aggregate stability

The paper investigates how Pseudomonas aeruginosa aggregates respond to host-derived stress, identifying a role for potassium homeostasis in aggregate stability and dispersal.

Gluconate acts as a signal to induce biofilm in Bacillus subtilis

This study identifies gluconate as a signal that induces biofilm formation in Bacillus subtilis by increasing iron availability, independent of bacillibactin.

COPAL: An ensemble of protein-ligand co-folding models enriches preferred ligand predictions for the LuxR-family of quorum sensing receptors

COPAL uses an ensemble of protein-ligand co-folding models to predict preferred AHL ligands for LuxR receptors, achieving higher accuracy than individual models.

High-resolution lineage tracking maps the multi-phase evolution and adaptive potential of biofilms

This study tracks lineage evolution in biofilms using DNA barcoding and spatial sampling, revealing phases of segregation, migration, and adaptation that influence antibiotic resistance.

BiofilmQ-HT: An integrated browser-based workflow for standardized analysis of high-throughput crystal violet-based biofilm assays

BiofilmQ-HT provides a standardized workflow for analyzing high-throughput crystal violet biofilm assays, improving data consistency and reproducibility across experimental conditions.

RsmW is an iron-responsive 3 UTR derived small regulatory RNA that contributes to biofilm formation and virulence of Pseudomonas aeruginosa

This study identifies RsmW as an iron-responsive sRNA that enhances biofilm formation and virulence in Pseudomonas aeruginosa under iron limitation.

Computational Structure Modeling, Functional Characterization, and Identification of Potential Inhibitors for the cyclic-di-GMP Mediated Biofilm Forming Membrane Protein in Vibrio cholerae

This paper identifies natural inhibitors of a membrane protein involved in biofilm formation in Vibrio cholerae through structural modeling, functional analysis, and virtual screening.

Pseudomonas aeruginosa Intra-Population Diversity Shapes Host Airway Epithelial Responses

The paper shows that diverse Pseudomonas aeruginosa populations elicit distinct host responses compared to individual variants, highlighting the importance of studying populations rather than single clones in chronic infections.

A Sensory Pathway that Controls Bacterial Zorbing

The paper identifies a sensory pathway in Flavobacterium johnsoniae that controls the transition between swarming and zorbing, linking environmental sensing to collective behavior and spatial organization.

Uncovering the mechanism of action of a historical antimicrobial against S. aureus and A. baumannii

This paper investigates the multifaceted antibacterial mechanisms of Bald's eyesalve against S. aureus and A. baumannii, showing its potential as a multi-target antimicrobial.

Polymicrobial catheter biofilms sustain susceptible Enterococcus faecalis and Escherichia coli during β-lactam treatment

The paper shows that susceptible Enterococcus faecalis and Escherichia coli in catheter biofilms survive β-lactam treatment through mutualistic interactions and persister-like states, without resistance.

Identification and structural basis of a Chloroflexus protein with homology to Bacillus quorum sensing-related prenyltransferase

The paper identifies a ComQ homolog from Sphaerobacter thermophilus and determines its structural basis for peptide prenylation, revealing insights into the evolution of this modification within the IPPS superfamily.

MucD regulates alginate biosynthesis through the proteolytic control of AlgX and AlgK in Pseudomonas aeruginosa

This study identifies MucD as a protease that regulates alginate biosynthesis in Pseudomonas aeruginosa by degrading AlgX and AlgK, revealing its post-translational control mechanism.

Diversification of biofilm architecture among freshwater Pararheinheimera isolates

The paper investigates ALI colonization by freshwater Pararheinheimera bacteria, identifying diverse biofilm architectures including traditional pellicles and buoyant hydrogels.

Disrupting chemotaxis stimulates adhesion by recruiting a putative c-di-GMP effector to the Caulobacter crescentus cell pole

This study identifies cmrA as a key regulator of hyperadhesion in C. crescentus chemotaxis mutants by recruiting it to the cell pole to activate PleD.

Within-host population structure, migration, and parallel adaptive evolution of Pseudomonas aeruginosa in cystic fibrosis lung disease

This study analyzes 450 Pseudomonas aeruginosa isolates from CF patients to show structured population dynamics, migration, and parallel adaptive evolution within lung infections.

Redox-dependent lipophilicity of phenazine metabolites is modulated by intramolecular hydrogen bonds and controls their biological distribution

This study measures the redox-dependent lipophilicity of phenazines and shows how their redox state affects retention in bacterial cells.

Enterococcus faecalis biofilm rewires neutrophil metabolism to suppress antimicrobial activity

This study shows E. faecalis biofilms suppress neutrophil defenses by producing lactic acid and high bacterial density.

Clonal microcolonies recruit individual planktonic colonizers using extracellular matrix factors to assemble Vibrio cholerae pellicles

This study shows how Vibrio cholerae pellicles form through planktonic cell recruitment mediated by extracellular matrix factors and quorum-sensing.