Bacterial biofilms and quorum sensing

A natural glycolipid exposes the outer membrane interface as a tunable regulator of bacterial surface attachment.

This study identifies dalberoside, a glycolipid that inhibits bacterial surface attachment and biofilm formation by remodeling the outer membrane interface without killing bacteria.

Biofilm initiation associated with cell orientation patterning in Escherichia coli

The paper shows that cell orientation patterning in Escherichia coli is linked to the spatial activation of the Rcs stress-response pathway, which is involved in extracellular matrix production and biofilm initiation.

Comparison of Galleria mellonella, Epithelial Cell Cytotoxicity, and Mouse Model of Bacteremia to Measure Pseudomonas aeruginosa Virulence

This study compares Galleria mellonella, epithelial cell cytotoxicity, and mouse models for measuring Pseudomonas aeruginosa virulence, finding G. mellonella correlates better with mouse results for population-level trends.

Flow-Dependent CFU Dynamics Reshape Polymicrobial Biofilm with a Pronounced Dominance Shift under Sub-Inhibitory Antibiotic Stress

This study shows how continuous flow and sub-inhibitory antibiotics alter biofilm dynamics and species dominance in polymicrobial communities on catheters.

Canonical pathoadaptive cystic fibrosis genes in Pseudomonas aeruginosa are not CF-specific

The paper evaluates the specificity of canonical Pseudomonas aeruginosa genes associated with cystic fibrosis (CF) by comparing their mutational patterns across diverse clinical and environmental contexts.

Cultivation-dependent effects of quorum sensing signals on a lactic acid and chain-elongating bacterium

This study evaluates how quorum sensing signals affect two microbial species in pure and co-cultures, showing context-dependent responses that are not predictable from pure-culture assays.

Analysis of the DNA-binding domain of the Pseudomonas aeruginosa quorum sensing transcription factor LasR

The paper investigates mutations in the DNA-binding domain of LasR, a LuxR homolog in Pseudomonas aeruginosa, finding some variants enhance regulation of RhlR-target genes but confer fitness defects.

Tetrasodium EDTA disrupts Pseudomonas aeruginosa membrane integrity, shows suppressed resistance evolution and reduced cytotoxicity compared to meropenem

This study shows that tEDTA disrupts Pseudomonas aeruginosa membranes, suppresses resistance evolution, and has lower cytotoxicity than meropenem.

Highly effective modulator therapy hinders the emergence of hyperbiofilm variants of Pseudomonas aeruginosa PA14 grown in a cystic fibrosis lung model

This study shows that elexacaftor/tezacaftor/ivacaftor (ETI) reduces biofilm formation and small colony variants in Pseudomonas aeruginosa PA14 grown in a CF lung model.

High-throughput dual bioreporter screening reveals distributed regulation of biofilm matrix components in Staphylococcus aureus

This paper develops a high-throughput bioreporter screening method to study biofilm regulation in S. aureus, revealing distinct regulatory networks for adhesion and extracellular DNA production and identifying mntR as a shared regulator.

Extracellular membrane vesicles - previously unrecognized components of Staphylococcus aureus biofilms

This study identifies extracellular membrane vesicles (MVs) as key contributors to Staphylococcus aureus biofilm formation by carrying matrix components like proteins and DNA.

Atypical BlaIR Two-Component System in Pseudomonas aeruginosa Regulates Virulence but not β-Lactam Resistance

This study identifies an atypical BlaIR system, pvmSR, in Pseudomonas aeruginosa that regulates virulence but not β-lactam resistance, using C. elegans as a model.

Structure of a key adhesin-exopolysaccharide interaction provides insights into matrix assembly in Vibrio cholerae biofilms

This study determines the structure of Bap1 bound to VPS and shows how it facilitates biofilm adhesion and VPS organization through a conserved β-propeller interaction.

Pharmacodynamic modeling of colistin and imipenem against in vitro Pseudomonas aeruginosa biofilms

This study develops a PK-PD model for imipenem and colistin against Pseudomonas aeruginosa biofilms to optimize clinical dosing schedules.

Universal-Bac3Gel: a 3D Biofilm-Relevant Matrix that Supports In Vitro Growth and Biofilm Formation of ESKAPE Pathogens.

This paper evaluates Universal-Bac3Gel as a 3D matrix for studying ESKAPE pathogens, showing successful bacterial growth and species-specific biofilm formation.

Copper as a central element critical to interkingdom interactions within a polymicrobial wound environment.

This study identifies copper homeostasis as a key factor in S. aureus competitiveness within polymicrobial wound infections, linking copA mutations to wound-based infections.

Bacteria Hijack a Host Metabolite via Three Distinct Sensors to Orchestrate Infection

This study shows bacteria use three distinct sensors to detect a host metabolite and coordinate infection, with a drug targeting one sensor showing high efficacy against resistant pathogens.

Catabolite Activator Protein and quorum sensing cross-control group behaviors in Vibrio campbellii

This study identifies how CAP and LuxR co-regulate gene expression in Vibrio campbellii through synergistic or antagonistic promoter occupancy, revealing indirect regulation of bioluminescence.

Functional impact of a type VI secretion system on RsmE-mediated spatial structure formation in Pseudomonas fluorescens Pf0-1

The paper identifies a type VI secretion system regulated by RsmE in Pseudomonas fluorescens Pf0-1 that kills invading WT cells in low-density spatiogenetic structures, but its effectiveness depends on co-produced extracellular secretions.

Inhibition of Pseudomonas aeruginosa-secreted protease IV reduces lung

This study identifies PrpL as a key Pseudomonas aeruginosa protease that activates AP-1 and drives lung inflammation, and shows that inhibiting PrpL reduces cytokine production and clinical symptoms.

Distribution of luxS and production of autoinducer-2 among gut Bacteroidales

This study investigates the distribution of luxS and AI-2 production in gut Bacteroidales, showing that many species produce AI-2 but do not respond to it.

Utilization of cyanobacterial siderophore cyanochelin B by phylogenetically distant heterotrophs suggest its role in mediating microbial interactions

The paper shows that cyanobacterial siderophore cyanochelin B is used by distant heterotrophs under iron limitation, supporting microbial interactions in soil crust ecosystems.

A genome-wide screen in Pseudomonas aeruginosa identifies genes impacting production of the hemolytic phospholipase C/sphingomyelinase, PlcH

This study identifies genes affecting PlcH production in Pseudomonas aeruginosa using a genome-wide transposon screen and confirms their roles through deletion mutants.

Development of low cost, robust and reproducible biofilm static and pharmacodynamic assays

The paper develops a low-cost, robust, and reproducible method for static and pharmacodynamic biofilm assays using glass beads and standard lab equipment, showing comparable results to traditional methods.

A switch-rheostat circuit governs quorum sensing homeostasis in a phytopathogen

This study identifies a regulatory switch and modulator in Pseudomonas fuscovaginae that control quorum sensing activation and output, revealing a two-tiered repression mechanism.

Multi-omics profiling reveals convergent adaptation of urinary Pseudomonas aeruginosa isolates

The paper analyzes multi-omics data from urinary Pseudomonas aeruginosa isolates to show convergent metabolic and phenotypic adaptations during urinary tract colonization.

The Testing and Characterization of the Bacterial Response to Novel Lipid Ether Amine-Based Antibiotics

This study tests novel lipid ether amine-based antibiotics against biofilm bacteria, showing they disrupt lipid metabolism and reduce biofilm formation more effectively than natural fatty acids.

Cyclic-Phe-Pro Binds the ToxRS Interface to Promote Signal Transduction in Vibrio vulnificus

This study identifies how cFP binds ToxRS in V. vulnificus to activate virulence genes by stabilizing the ToxR-ToxS heterodimer and enhancing promoter binding.

A Two-Component Regulatory System Mediates Quorum Sensing-Dependent Morphology and Motility Transitions in the Archaeon Haloferax volcanii

Azelaic Acid as a Novel Antivirulence Agent Against Pseudomonas aeruginosa: In silico, In vitro and In vivo Efficacy and Biocompatibility

This study shows azelaic acid reduces Pseudomonas aeruginosa virulence factors without killing bacteria, with safety and molecular docking support.