Zebrafish Biomedical Research Applications

This study identifies diencephalic and neuropeptidergic dysfunction in zebrafish with autism-risk mutations, revealing potential molecular and behavioral mechanisms.

This paper introduces a new clearing reagent, LUCID, enabling high-resolution 3D imaging of whole zebrafish, including dense tissues like bone and cartilage, without significant distortion or signal loss.

The paper develops a multi-behavioral phenotyping approach in larval zebrafish to identify neurotoxic chemicals, finding chlorophene disrupts habituation via GABAARs and resembles Kv7 activator effects.

This study characterizes a zebrafish ebf3a mutant, revealing brain size reduction, functional impairments, and gene expression changes in sensory systems and the cerebellum.

This paper develops an embryonic zebrafish xenograft model to assess the efficacy of multi-receptor tyrosine kinase inhibitors in rhabdomyosarcoma, identifying VEGF-A as a potential biomarker for Regorafenib response.

The paper characterizes four existing and three new transgenic lines in larval zebrafish to label reticulospinal neurons and other brainstem neurons, providing genetic access for functional studies.

This study creates a zebrafish model of NAPB knockout to study autism and epilepsy, finding hyperactivity and hyperexcitability resembling human epileptic encephalopathy.

This paper develops an automated microinjection robot for zebrafish xenograft models, achieving 60% success rate and 70% survival rate, with twice the speed of manual injections.

The paper identifies a new external immune organ in zebrafish, the axillary lymphoid organ, suitable for live imaging of immune cell dynamics.

The paper develops a zebrafish model to predict breast cancer patient response to chemotherapy, showing perfect correlation between patient and model treatment outcomes.

The paper shows that optimistic and pessimistic cognitive biases in zebrafish affect stress reactivity and cancer progression, with optimists showing lower tumorigenesis.

This paper presents a dataset documenting zebrafish early development at 26°C and 28°C, showing temperature-dependent developmental milestones and suggesting revised guidelines for toxicological studies.

This study shows that social status in zebrafish affects their behavioral response to 5-HT2C receptor agonists and antagonists in a novel tank test.

The paper develops a pipeline for whole-brain activity mapping in adult zebrafish and identifies a novel functional connectome during the novel tank test.

A structured zebrafish embryo toxicity database (INTOB, 638 experiments) enabled control-quality tests, dose-response modeling and phenotype-fingerprint clustering of 47 substances (133 experiments); literature data proved unsuitable for meta-analysis.

This study maps phosphoproteomic changes during zebrafish caudal fin regeneration, identifying key signaling pathways and highlighting the role of phosphorylation in tissue regeneration.

Marigold is a web-based tool for zebrafish behavior analysis that enables high-throughput tracking of pose and kinematic parameters across diverse experimental setups.

This study uses zebrafish crispant screening to functionally validate disease-causing genes for bone fragility disorders, showing consistent skeletal phenotypes and differential gene expression in affected models.

This study shows that serotonergic signaling in zebrafish modulates the shift from orienting to defensive responses when exposed to alarm cues.

This paper develops a zebrafish xenograft model for diffuse midline glioma by transplanting human DMG cells into zebrafish embryos to enable high-throughput drug screening.

The paper compares sensorimotor responses in wild-type and GCaMP6s transgenic zebrafish larvae, finding a developmental switch in light and vibration responses but no major difference in response maturation.

The paper develops an automated image processing tool to quantify bacterial burdens in zebrafish larvae by detecting their borders to distinguish internal from external bacteria.

This study identifies three FDA-approved drugs that rescue zebrafish from sepsis-like endotoxicity with no neurobehavioral side effects.

This study maps transsynaptic connections in zebrafish neural circuits using a modified trans-Tango method and validates findings with optogenetic experiments.

This study generates and characterizes fgfr4 knockout zebrafish to investigate FGFR4's role in development and disease.

This study shows environmental enrichment reduces anxiety and attention deficits but not impulsivity in adgrl3.1 knockout zebrafish, indicating genetic and environmental interactions in behavioral traits.

This study shows that the size and position of the zebrafish swim bladder are physically constrained by surrounding bones during development.

The paper develops a method to analyze behavioral variability in larval zebrafish by identifying motor strategies and their transitions across different environmental cues.

The paper examines how social isolation interacts with a genetic mutation to intensify both externalizing and internalizing behaviors in zebrafish.