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This study investigates how intron RNA structure affects gene expression using reporter assays and machine learning, finding that base pairing significantly modulates mRNA and protein levels.

The paper identifies SPRY2 as a novel K-Ras effector that impacts its membrane organisation through proteomics and BRET screens.

This study identifies Nischarin S-glutathionylation as a redox-regulated mechanism controlling cell migration and invasion in breast cancer by modulating Rac1 and PAK1 interactions.

The paper optimizes conditions for GTP loading of H-Ras by evaluating factors like time, temperature, concentration, and mutations to enhance nucleotide exchange efficiency.

This study develops a dimeric curvature-sensing peptide that enhances sEV cellular uptake and drug loading, showing fivefold and 20fold increases in uptake and peptide accumulation, respectively.

This study determines the cryo-EM structures of HSV-1 helicase-primase complex with and without inhibitors, revealing how pritelivir and amenamevir block helicase activity.

The paper discovers and characterizes a new family of bacterial heme-thiolate peroxygenases, showing structural and functional similarities to eukaryotic UPOs.

This study identifies and characterizes a small-molecule inhibitor, A12, of the SOS response in Pseudomonas aeruginosa, showing it binds RecA and LexA and forms disulfide bonds, with potential for anti-SOS compound development.

The paper develops a microvolume method for measuring catalase activity using a NanoDrop spectrophotometer, enabling efficient analysis with minimal sample volumes.

This study analyzes the catalytic domains of D29LysA and DS6ALysA phage endolysins to understand their role in mycobacterial cell-wall hydrolysis and their potential as therapeutic agents.

The paper identifies cysteine S-acetylation as a novel post-translational modification in mouse liver, revealing its cytoplasmic localization and tissue-specific patterns under metabolic stress.

This paper introduces a high-throughput spatial proteomics workflow for FFPE samples, enabling efficient processing and analysis of subcellular regions with high resolution.

This study identifies proteins in tear fluid associated with malignant transformation of oral lesions, suggesting tear fluid as a noninvasive liquid biopsy for early detection.

This study identifies NUDT5 as a regulator of purine metabolism and thiopurine sensitivity by interacting with PPAT, independent of its known enzymatic activity.

The paper identifies CDK8/19 inhibitors with reduced off-target toxicity and shows that their loss is not cytotoxic but reduces cell proliferation.

The paper solves high-resolution structures of TXNL1 bound to the human 26S proteasome, revealing conformation-specific binding and new insights into ATP hydrolysis and substrate translocation mechanisms.

The paper shows hypoxia-induced mitochondrial ROS leads to M1 ubiquitin chains, activating NF-κB signaling and increasing target gene expression in response to mitochondrial stress.

This study characterizes a ubiquitin E3 RING ligase from Simkania negevensis, showing it generates K63- and K11-linked ubiquitin chains and interacts with specific E2 ligases.

This study determines high-resolution structures of immature HIV-1 particles with inhibitors BVM and LEN bound using cryo-EM, revealing their distinct binding mechanisms and effects on Gag lattice architecture.