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The paper identifies a novel ferredoxin-NADP+ reductase in C. pasteurianum, characterizes its structure and function, and reveals its role in redox regulation during sporulation.

This paper determines the cryo-EM structure of the PI3KC3-C2 complex with Rubicon's PIKBD domain, revealing how Rubicon selectively inhibits PI3KC3-C2 to regulate endolysosome maturation and mitophagy.

This paper implements Gaussian accelerated molecular dynamics in GROMACS, showing improved sampling of conformational and binding free-energy basins compared to conventional MD simulations.

The paper develops a chemoselective bioconjugation method to detect and analyze NEylation, revealing it as a widespread PTM affecting protein function in cells.

This study quantitatively profiles JMJD6-catalyzed lysine hydroxylation, revealing a graded, residue-dependent response to oxygen availability in lysine-rich regions.

The paper identifies TRPM8 as an ion channel regulated by phospholipid asymmetry and shows that cytoplasmic phosphatidylserine is required for its function.

This study identifies SPAR, a microprotein, as an inhibitor of SLC38A9, a lysosomal amino acid transporter, revealing its role in mTORC1 pathway regulation by blocking arginine transport and Rag GTPase recruitment.

This paper identifies and characterizes the sinefungin biosynthetic gene cluster, revealing unconventional biocatalytic strategies involving a B12-dependent radical SAM enzyme and PEARLs for nucleoside modification.

The paper identifies convergent biosynthetic pathways for 20-hydroxyecdysone in spinach, quinoa, and Ajuga, revealing distinct gene clusters and evolutionary convergence.

This study identifies conformational signatures of non-retinoid ligands and 11-cis-retinal binding to rhodopsin using multiple techniques, revealing differential stabilization mechanisms.

This study identifies HAX1 as a stimulatory cofactor for CLPB, showing it promotes CLPB oligomerization and enhances its ATPase and refoldase activities.

This study identifies siderophores from Actinoplanes teichomyceticus with antibacterial properties, showing activity against Gram-positive bacteria through a metal-dependent mechanism.

This study reports the crystal and cryo-EM structures of Streptococcus pneumoniae NDK, revealing its hexameric form and higher-order supramolecular assemblies, and suggests these structures may modulate its function.

This study identifies bL27's role in preventing tmRNA from interfering with protein synthesis during trans-translation in bacteria.

The paper identifies aliphatic-to-threonine substitutions as a conserved adaptation in mitochondrial membrane proteins that balances hydrophilicity and hydrophobicity requirements.

The paper identifies CEM198 as a TTL binder through chemoproteomics, showing it disrupts microtubule dynamics via dual mechanisms in different cell lines.

This study identifies acid phospholipase A1's role in lysosomal glycerophospholipid catabolism and shows its interaction with bis(monoacylglycero)phosphate and inhibition by cationic amphiphilic drugs.

The paper introduces tinyRNA-seq, a method to sequence very short RNAs with reduced bias, and shows it outperforms commercial kits in capturing small RNA sequences.

This study shows that regulatory performance of inducible and co-repressible systems depends on how ligand binding redistributes conformational states, with natural co-repressible regulators performing better.

This paper designs a de novo protein fold that binds doxorubicin with high affinity through aromatic π-stacking, revealing a new 5-helix globular fold.