Discover, rate, and discuss biology preprints.

The paper proposes the Kinetic Intron Hypothesis to model intron length by exploring hypothetical dynamics of intron RNA synthesis and degradation, presenting preliminary data on novel intron characteristics.

The paper determines the structure of the Gβγ-prefusion SNARE complex using cryo-EM and proposes a model for how Gβγ inhibits synaptic vesicle fusion by blocking SNARE complex assembly.

The paper quantifies the kinetic hierarchy of Kai protein complex formation in cyanobacteria, revealing distinct assembly dynamics that regulate the circadian oscillator.

The paper develops a framework to train supervised models on MAVE data by accounting for experimental protocol differences, improving variant effect prediction across datasets.

The paper develops a mathematical model to quantify how Golgi cisterna morphology affects glycosylation efficiency, identifying optimal and maximal thicknesses for successful polymer production.

The paper uses free-energy perturbation to calculate binding affinities of cAMP to HCN isoforms 1-4 CNBDs, revealing differences in channel activation sensitivity.

This study characterizes the molecular interfaces of the SARS-CoV-2 N protein's CTD and flanking IDRs, revealing their roles in oligomerization, RNA binding, and phase separation.

This study shows chlorpromazine inhibits EAG1 channels by disrupting PAS-CNBH domain coupling, without affecting VSD movement.

This paper determines the solution structure of the CH-domain zinc finger from Makorin-3, revealing its metal-binding properties and potential role in protein interactions.

This paper engineers OAA lectins to selectively bind specific high mannose glycans, enhancing sensitivity and applicability in glycan profiling and antiviral applications.

This paper develops a novel cardiomyocyte pair model to quantify gap junction and ephaptic contributions to electrical conduction, showing that ephaptic mechanisms dominate at physiological sodium levels.

This paper provides a protocol for building and characterizing an oblique plane microscope (OPM) to enable fast, multi-color, high-resolution volumetric fluorescence imaging with reduced phototoxicity.

This study uses microsecond MD simulations and FEP to analyze nucleotide binding and conformational dynamics of PCAT1, revealing Mg2+ and substrate-dependent stabilization mechanisms.

This study investigates how calmodulin mutations affect voltage-gated calcium channel regulation in neurons, linking these effects to neurological symptoms in calmodulinopathies.

This study examines how single-residue substitutions in a mitochondrial localization peptide affect its conformational dynamics, revealing that sequence changes can bias structural ensembles and influence targeting efficiency.

The paper investigates how tau's liquid-liquid phase separation affects its binding to BRICHOS and tubulin, showing conformational changes regulate these interactions.

The paper introduces HXMS, a standardized file format for HX-MS data, and provides PFLink to convert existing data to this format, enhancing data processing and sharing.

This paper introduces a nanopatterning toolbox to study multiprotein assemblies in live cells, demonstrating its application to the myddosome complex and revealing nanoscale organization.

Chart Builder provides an interactive tool for visualizing and exploring metadata from cryoEM archives, enabling users to create customizable charts and access underlying datasets.

This study investigates the McdAB system in Halothiobacillus neapolitanus, revealing that McdA's ATPase activity is DNA-stimulated and that McdB displaces McdA from DNA, forming gradients to prevent carboxysome aggregation.