A. E. Cohen

Ether lipids influence cancer cell fate by modulating iron uptake

This study shows that ether lipids in cancer cells regulate membrane properties to influence iron uptake, ferroptosis, and metastasis, offering a therapeutic target.

Mapping uterine calcium dynamics during the ovulatory cycle in live mice

The paper maps uterine calcium dynamics in live mice across the estrous cycle using a calcium reporter and imaging, revealing phase-dependent initiation patterns of uterine excitations.

Structural basis of transcription: RNA Polymerase II substrate binding and metal coordination at 3.0 A using a free-electron laser

This paper presents the structure of ATP-bound RNA Polymerase II at 3.0 Å resolution using a free-electron laser, revealing new insights into substrate binding and metal coordination in the active site.

Changes in an Enzyme Ensemble During Catalysis Observed by High Resolution XFEL Crystallography

The paper uses XFEL crystallography to observe conformational changes in an enzyme during catalysis, revealing ionization-linked dynamics that control catalytic activity.

All-optical mapping of cAMP transport reveals rules of sub-cellular localization

The paper measures cAMP diffusion in cells using an all-optical toolkit, finding a diffusion coefficient of ~130 µm²/s and domain length-scales of 27 µm in dendrites.

Diminishing neuronal acidification by channelrhodopsins with low proton conduction

The paper identifies and characterizes channelrhodopsins with low proton permeability to mitigate neuronal acidification during optogenetic stimulation.

Topological action potentials in engineered tissues

The paper demonstrates that gap junction-coupled interfaces between non-excitable tissues can exhibit electrical excitability, a topologically protected phenomenon with broader ion channel expression tolerance than bulk excitability.

The mechanisms of catalysis and ligand binding for the SARS-CoV-2 NSP3 macrodomain from neutron and X-ray diffraction at room temperature

This study determines the crystal structures of SARS-CoV-2 NSP3 macrodomain using neutron and X-ray diffraction, revealing insights into its catalytic mechanism and ligand binding.

Linearly polarized excitation enhances signals from fluorescent voltage indicators

This study shows that adjusting the polarization of excitation light can significantly enhance fluorescence signals from voltage indicators in mammalian cells, especially in thin neurites.

Fragment binding to the Nsp3 macrodomain of SARS-CoV-2 identified through crystallographic screening and computational docking

The paper identifies new chemical fragments binding to the SARS-CoV-2 Nsp3 macrodomain through crystallographic screening and computational docking, validating their binding with biophysical techniques.

Prednisolone rescues Duchenne Muscular Dystrophy phenotypes in human pluripotent stem cells-derived skeletal muscle in vitro

This study shows prednisolone rescues DMD phenotypes in iPSC-derived muscle cells, suggesting direct myofiber effects rather than just anti-inflammatory benefits.

Mix-and-inject XFEL crystallography reveals gated conformational dynamics during enzyme catalysis

This study uses mix-and-inject XFEL crystallography to observe conformational changes in isocyanide hydratase during catalysis, showing how cysteine modification affects enzyme dynamics and function.

Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

This study compares serial X-ray crystallography and MicroED for determining structures from small macromolecular crystals, highlighting their strengths and challenges.

Bioelectrical signaling via domain wall migration

The paper demonstrates bioelectrical domain wall migration as a novel form of long-range signaling in tissues.

Molecular mimicry in deoxy-nucleotide catalysis: the structure of Escherichia coli dGTPase reveals the molecular basis of dGTP selectivity

This paper determines the structure of Escherichia coli dGTPase and elucidates the molecular basis of its dGTP selectivity through comparative analysis with SAMHD1.

Geometry-dependent instabilities in electrically excitable tissues

The paper investigates how tissue geometry influences electrical dynamics in excitable cells through long-range electrical signaling and gap junction coupling.

Cell membranes resist flow

This study shows that membrane tension does not propagate across intact cells, challenging the idea that it mediates long-range signaling.