A. H. de Ru

Proteolytic activity of surface exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile

This study shows that HtrA proteolytic activity affects its expression level and is necessary for C. difficile survival under acidic conditions.

AMPK activation induces RALDHhigh tolerogenic dendritic cells through rewiring of glucose and lipid metabolism

This study shows that AMPK activation makes human dendritic cells more tolerogenic by altering their metabolism, specifically increasing RALDH activity and changing lipid and glucose metabolism.

In-depth specificity profiling of Pro-Pro endopeptidases (PPEPs) using combinatorial synthetic peptide libraries

The paper develops a method combining synthetic peptide libraries and mass spectrometry to profile the specificity of Pro-Pro endopeptidases (PPEPs) and identifies new substrates and specificity patterns.

Clostridioides difficile phosphoproteomics shows an expansion of phosphorylated proteins in stationary growth phase

Healthy cells functionally present TAP-independent SSR1 peptides: implications for selection of clinically relevant antigens

The paper identifies TAP-independent antigen presentation in healthy cells using a T cell clone and highlights the need for comprehensive testing of healthy cell types to safely target antigens for immunotherapy.

Site-specific glycosylation mapping of Fc gamma receptor IIIb from neutrophils of health donors

This study maps glycosylation sites of Fc gamma receptor IIIb from neutrophils using LC-MS/MS and identifies donor allotype differences.

PAKC: A novel Panel of HLA class I Antigen presentation machinery Knockout Cells from the same genetic origin

The paper generates a panel of APM knockout cell lines to study HLA class I antigen presentation and its role in immune responses.