D. P. Byrne

Redox Regulation of Brain Selective Kinases BRSK1/2: Implications for Dynamic Control of the Eukaryotic AMPK family through Cys-based mechanisms

This study investigates how redox regulation via cysteine oxidation controls BRSK1/2 kinase activity, revealing conserved mechanisms that modulate catalytic function through disulfide bond formation and salt bridge disruption.

A bespoke analytical workflow for the confident identification of sulfopeptides and their discrimination from phosphopeptides

This study develops a mass spectrometry workflow to identify sulfotyrosine peptides and distinguish them from phosphotyrosine peptides, identifying 21 new sulfotyrosine-containing proteins.

Mechanistic and evolutionary insights into isoform-specific 'supercharging' in DCLK family kinases

This study investigates how isoform-specific variations in DCLK family kinases affect autoregulation through structural and functional analyses.

Evolutionary and cellular analysis of the dark pseudokinase PSKH2

This study investigates the evolutionary and cellular roles of the pseudokinase PSKH2, confirming its catalytic inactivity and identifying its mitochondrial localization and chaperone-dependent regulation.

Exploring the conformational landscape and stability of Aurora A using ion-mobility mass spectrometry and molecular modelling

This study uses ion-mobility mass spectrometry and molecular modeling to analyze conformational changes in Aurora A kinase induced by TPX2 activation and inhibitor binding.

Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota

This study analyzes carbohydrate sulfatases from Bacteroides thetaiotaomicron, correlating structural and functional data with phylogenetic and environmental analyses to understand sulfated glycan metabolism.

KinOrtho: a method for mapping human kinase orthologs across the tree of life and illuminating understudied kinases

KinOrtho maps human kinase orthologs across 17,000 species, identifies understudied kinases, and proposes their functions using domain-based approaches and machine learning.

Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity.

This study develops a rapid capillary electrophoresis assay for quantifying carbohydrate desulfation by sulfatases, demonstrating its advantages over existing methods.

A single bacterial sulfatase is required for metabolism of colonic mucin O-glycans and intestinal colonization by a symbiotic human gut bacterium

This study identifies a single sulfatase essential for colonic mucin O-glycan metabolism and intestinal colonization by Bacteroides thetaiotaomicron.

Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signaling networks using SILAC-based phosphoproteomics

This study uses centrinone and SILAC-based phosphoproteomics to identify PLK4 substrates and reveal a network of PLK4-regulated phosphorylation motifs in human cells.

A novel redox-active switch in Fructosamine-3-kinases expands the regulatory repertoire of the protein kinase superfamily

This study identifies redox-sensitive cysteines in Fructosamine-3-kinases (FN3Ks) that regulate their activity and dimerization through disulfide bonds, revealing a novel redox regulatory mechanism in the protein kinase superfamily.

An evolutionary-conserved redox regulatory mechanism in human Ser/Thr protein kinases

The study identifies a conserved redox regulatory mechanism in human Ser/Thr kinases through cysteine oxidation in the activation segment.

New tools for carbohydrate sulphation analysis: Heparan Sulphate 2-O-sulphotranserase (HS2ST) is a target for small molecule protein kinase inhibitors

This paper introduces a new non-radioactive assay for HS2ST activity using a synthetic fluorescent substrate and identifies protein kinase inhibitors that inhibit HS2ST-catalyzed sulphation.