Author

P. A. Eyers

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 9 Evolutionary Biology· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Assay:proteomics· 10 Approach:experimental assay· 8 Organism:human· 7 Approach:statistical model· 5 Research Focus:cell type annotation· 4 Research Focus:protein folding & dynamics· 4 Research Focus:drug response / drug discovery· 3 Research Focus:gene regulatory network inference· 2

Frequent Co-Authors

D. P. Byrne· 9 shared C. E. Eyers· 6 shared L. A. Daly· 4 shared A. R. Jones· 3 shared N. Kannan· 3 shared A. Cartmell· 2 shared A. Venkat· 2 shared E. A. Yates· 2 shared

Activity by Year

2018-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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9 shared papersBiochemistry

D. P. Byrne

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Author profile →

11 papers

Recent·Most cited

Redox Regulation of Brain Selective Kinases BRSK1/2: Implications for Dynamic Control of the Eukaryotic AMPK family through Cys-based mechanisms

G. Bendzunas, D. P. Byrne, S. Shrestha, L. A. Daly, S. O. Oswald, S. Katiyar, A. Venkat, W. Yeung, C. E. Eyers, P. A. Eyers, N. Kannan

This study investigates how redox regulation via cysteine oxidation controls BRSK1/2 kinase activity, revealing conserved mechanisms that modulate catalytic function through disulfide bond formation and salt bridge disruption.

bioRxiv · Oct 5, 2023 · PreprintBiochemistry

A bespoke analytical workflow for the confident identification of sulfopeptides and their discrimination from phosphopeptides

L. A. Daly, D. P. Byrne, S. Perkins, P. J. Brownridge, E. McDonnell, A. R. Jones, P. A. Eyers, C. E. Eyers

This study develops a mass spectrometry workflow to identify sulfotyrosine peptides and distinguish them from phosphotyrosine peptides, identifying 21 new sulfotyrosine-containing proteins.

bioRxiv · Jul 15, 2023 · PreprintBiochemistry

A Peroxiredoxin-P38 MAPK scaffold increases MAPK activity by MAP3K-independent mechanisms

M. Cao, A. M. Day, M. Galler, H. R. Latimer, D. Byrne, E. Bennett, E. Dwyer, P. A. Eyers, E. A. Veal

This paper shows that a Peroxiredoxin-P38 MAPK fusion protein hyperactivates P38 MAPK in yeast and human cells without requiring H2O2-sensing cysteines, suggesting a novel scaffold-based signaling mechanism.

bioRxiv · Nov 15, 2022Biochemistry

Evolutionary and cellular analysis of the dark pseudokinase PSKH2

D. P. Byrne, S. Shrestha, L. A. Daly, V. Marensi, K. Ramakrishnan, C. E. Eyers, N. Kannan, P. A. Eyers

This study investigates the evolutionary and cellular roles of the pseudokinase PSKH2, confirming its catalytic inactivity and identifying its mitochondrial localization and chaperone-dependent regulation.

bioRxiv · Sep 10, 2022Biochemistry

Exploring the conformational landscape and stability of Aurora A using ion-mobility mass spectrometry and molecular modelling

L. J. Tomlinson, M. Bachelor, D. P. Byrne, J. Sarsby, P. J. Brownridge, R. Bayliss, P. A. Eyers, C. E. Eyers

This study uses ion-mobility mass spectrometry and molecular modeling to analyze conformational changes in Aurora A kinase induced by TPX2 activation and inhibitor binding.

Journal of the American Society for Mass Spectrometry · Jan 31, 2022Peer-reviewedBiochemistry

Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota

A. S. Luis, A. Basle, D. P. Byrne, G. S. Wright, J. London, J. Chunsheng, N. Karlsson, G. C. Hansson, P. A. Eyers, M. Czjzek, T. Barbeyron, E. A. Yates, E. C. Martens, A. Cartmell

This study analyzes carbohydrate sulfatases from Bacteroides thetaiotaomicron, correlating structural and functional data with phylogenetic and environmental analyses to understand sulfated glycan metabolism.

bioRxiv · Jul 23, 2021Biochemistry

KinOrtho: a method for mapping human kinase orthologs across the tree of life and illuminating understudied kinases

L.-C. Huang, R. Taujale, N. Gravel, A. Venkat, W. Yeung, D. P. Byrne, P. A. Eyers, N. Kannan

KinOrtho maps human kinase orthologs across 17,000 species, identifies understudied kinases, and proposes their functions using domain-based approaches and machine learning.

bioRxiv · Mar 5, 2021Evolutionary Biology

Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity.

D. P. Byrne, J. A. London, P. A. Eyers, E. A. Yates, A. Cartmell

This study develops a rapid capillary electrophoresis assay for quantifying carbohydrate desulfation by sulfatases, demonstrating its advantages over existing methods.

bioRxiv · Dec 1, 2020Biochemistry

Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets with therapeutic potential

B. Meyer, J. Chiaravalli, S. Gellenoncourt, P. Brownridge, D. P. Bryne, L. A. Daly, A. Grauslys, M. Walter, F. Agou, L. A. Chakrabarti, C. S. Craik, C. E. Eyers, P. A. Eyers, Y. Gambin, A. R. Jones, , E. Emmott

This study identifies novel SARS-CoV-2 protease cleavage sites and cellular targets using mass spectrometry, showing that targeting these proteins can inhibit viral replication.

bioRxiv · Sep 16, 2020Microbiology

Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signaling networks using SILAC-based phosphoproteomics

D. P. Byrne, C. J. Clarke, P. Brownridge, A. Kalyuzhnyy, S. Perkins, A. Campbell, D. Mason, A. R. Jones, P. A. Eyers, C. E. Eyers

This study uses centrinone and SILAC-based phosphoproteomics to identify PLK4 substrates and reveal a network of PLK4-regulated phosphorylation motifs in human cells.

bioRxiv · May 22, 2020Biochemistry

New tools for carbohydrate sulphation analysis: Heparan Sulphate 2-O-sulphotranserase (HS2ST) is a target for small molecule protein kinase inhibitors

D. P. Byrne, Y. Li, K. Ramakrishnan, I. Barsukov, E. Yates, C. Eyers, D. Papy-Garcia, S. Chantepie, V. Pagadala, J. Liu, C. Wells, D. Drewry, W. Zuercher, N. Berry, D. Fernig, P. A. Eyers

This paper introduces a new non-radioactive assay for HS2ST activity using a synthetic fluorescent substrate and identifies protein kinase inhibitors that inhibit HS2ST-catalyzed sulphation.

bioRxiv · Apr 6, 2018Biochemistry